Monastrol

Name: Monastrol
Brand: Tocris Bioscience
SKU: 1305

Catalog # 1305 | Tocris Bioscience a Bio-Techne Brand

Citations (19)

COA / SDS

Selective inhibitor of mitotic kinesin Eg5

Key Product Details

Description:	Selective inhibitor of mitotic kinesin Eg5
Chemical Name:	1,2,3,4-Tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-5-pyrimidinecarboxylic acid, ethyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	292.35

View Full Technical Details for Monastrol

Technical Data
Citations (19) / Reviews

Biological Activity for Monastrol

Monastrol is a potent, cell-permeable, small molecule mitosis inhibitor that does not interact with tubulin. Arrests cells in mitosis and specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein required for mitotic spindle formation and maintenance (IC₅₀ = 14 μM).

Licensing Information

Sold with the permission of Harvard University

Compound Libraries for Monastrol

Monastrol is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Monastrol

M. Wt	292.35
Formula	C₁₄H₁₆N₂O₃S
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	254753-54-3
PubChem ID	5353454
InChi Key	UAIYNMRLUHHRMF-AATRIKPKSA-N
Smiles	CCOC(=O)C1=C(C)NC(=S)NC1C1=CC(O)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Monastrol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	5.85	20
	DMSO	29.24	100

Preparing Stock Solutions for Monastrol

The following data is based on the product molecular weight 292.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.42 mL	17.10 mL	34.21 mL
5 mM	0.68 mL	3.42 mL	6.84 mL
10 mM	0.34 mL	1.71 mL	3.42 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

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References for Monastrol

References are publications that support the biological activity of the product.

Kapoor Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J.Cell Biol. 2000 PMID: 10973989
Mayer Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 1999 PMID: 10542155

Citations for Monastrol (19)

Showing 1 - 10 of 19 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Monastrol include:

Drpic et al. (2018) Chromosome Segregation Is Biased by Kinetochore Size. Curr Biol. 28:1344
PMID: 29706521
Wong et al. (2018) Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment. Cell Death Discov. 4:109
PMID: 30510774
Shrestha (2017) Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells. Nat Commun. 8(1):150
PMID: 28751710
Asteriti et al. (2017) Identification of small molecule inhibitors of the Aurora-A/TPX2 complex. Oncotarget. 8:32117
PMID: 28389630
Floriot et al. (2015) C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle. Cell Cycle. 6:6894
PMID: 25902731
Barr and Bakal (2015) A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly. Nat Commun. 5:10564
PMID: 26037491
Asraf et al. (2015) Mitotic slippage and expression of survivin are linked to differential sensitivity of human cancer cell-lines to the Kinesin-5 inhibitor monastrol. PLoS One. 10:e0129255
PMID: 26035434
Shrestha and Draviam (2013) Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK. Curr Biol. 23:1514
PMID: 23891108
Corrigan et al. (2013) Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance. Springerplus. 12:2643
PMID: 23907121
Habu and Matsumoto (2013) p31(comet) inactivates the chemically induced Mad2-dependent spindle assembly checkpoint and leads to resistance to anti-mitotic drugs. Nature. 2:562
PMID: 24255856
Kabeche and Compton (2013) Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. J Biol Chem. 502:110
PMID: 24013174
Vladimirou et al. (2013) Nonautonomous movement of chromosomes in mitosis. Dev Cell. 27:60
PMID: 24135231
Lera and Burkard (2012) High mitotic activity of Polo-like kinase 1 is required for chromosome segregation and genomic integrity in human epithelial cells. J Biol Chem. 287:42812
PMID: 23105120
Bartoli et al. (2011) Kinesin molecular motor Eg5 functions during polypeptide synthesis. Mol Biol Cell. 22:3420
PMID: 21795388
Bassett et al. (2010) Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors. Sci Rep. 190:177
PMID: 20643881
Samoshkin et al. (2009) Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation. PLoS One. 4:e6831
PMID: 19714251
Vijapurkar et al. (2007) Potentiation of kinesin spindle protein inhibitor-induced cell death by modulation of mitochondrial and death receptor apoptotic pathways. Cancer Res. 67:237
PMID: 17210704
Chin (2006) Induction of apoptosis by monastrol, an inhibitor of the mitotic kinesin Eg5, is independent of the spindle checkpoint. Mol Cancer Ther. 5:2580
PMID: 17041103
Trinkle-Mulcahy et al. (2003) Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle. Mol Biol Cell. 14:107
PMID: 12529430

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