ML T7

Name: ML T7
Brand: Tocris Bioscience
SKU: 8119

Tocris Bioscience, part of Bio-Techne | Catalog # 8119

COA / SDS

Inhibitor of phosphatidylserine/CEACAM1 binding to Tim-3

Key Product Details

Description:	Inhibitor of phosphatidylserine/CEACAM1 binding to Tim-3
Chemical Name:	5-(2,4-Dichlorophenyl)-3a,6a-dihydro-3-(4-methylphenyl)spiro[1H-furo[3,4-c]pyrrole-1,2'-[2H]indene]-1',3',4,6(3H,5H)-tetrone
Purity:	≥98% (HPLC)
Molecular Weight:	506.34

View Full Technical Details for ML T7

Technical Data
Citations

Biological Activity for ML T7

ML T7 is a T cell immunoglobulin and mucin-containing molecule 3 (Tim-3) inhibitor; disrupts phosphatidylserine/CAECAM1 binding to Tim-3 (K_d = 7.0 μM and 7.4 μM for hTim-3 and mTim-3 respectively). ML T7 promotes cell proliferation, cytokine production and cytotoxicity of OT-I CD8+ cytotoxic T lymphocytes. ML T7 inhibits tumor growth in a syngeneic mouse model of hepatocellular carcinoma, increases NK cell tumor killing activity and DC maturation.

Technical Data for ML T7

M. Wt	506.34
Formula	C₂₇H₁₇Cl₂NO₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	459789-75-4
PubChem ID	3116616
InChi Key	OFJOQMMYCMPPEC-UHFFFAOYSA-N
Smiles	ClC1=CC=C(C(Cl)=C1)N2C(C(C3C2=O)C(OC43C(C5=C(C4=O)C=CC=C5)=O)C6=CC=C(C=C6)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML T7

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	50.63	100

Preparing Stock Solutions for ML T7

The following data is based on the product molecular weight 506.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.97 mL	9.87 mL	19.75 mL
5 mM	0.39 mL	1.97 mL	3.95 mL
10 mM	0.20 mL	0.99 mL	1.97 mL
50 mM	0.04 mL	0.20 mL	0.39 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for ML T7

References are publications that support the biological activity of the product.

Ma Identification of a small-molecule Tim-3 inhibitor to potentiate T cell-mediated antitumor immunotherapy in preclinical mouse models. Sci.Transl.Med. 2023 PMID: 37967204