ML 210
Tocris Bioscience, part of Bio-Techne | Catalog # 6429
Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis
Key Product Details
Description
Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis
Product Description
ML 210 is a glutathione peroxidase (GPX4) inhibitor. Kills mutant RAS-expressing cell lines (IC50 values are 71 and 272 nM for HRAS G12v mutant expressing cell lines BJeLR and BJeH-LT, respectively). Exhibits 4-fold selectivity for HRAS mutant-expressing cell lines. Induces ferroptosis in tumor initiation "persister" cells and drug-resistant tumor cells.Product Specifications
Molecular Weight
475.32
Formula
C22H20Cl2N4O4
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
[4-[Bis(4-chlorophenyl)methyl]-1-piperazinyl](5-methyl-4-nitro-3-isoxazolyl)methanone
CAS Number
1360705-96-9
PubChem ID
49766530
InChI Key
VIBHJPDPEVVDTB-UHFFFAOYSA-N
SMILES
CC1=C([N+]([O-])=O)C(C(N2CCN(C(C3=CC=C(C=C3)Cl)C4=CC=C(C=C4)Cl)CC2)=O)=NO1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 23.77 | 50 |
Preparing Stock Solutions
for ML 210
The following data is based on the product molecular weight 475.32
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.21 mL | 21.04 mL | 42.08 mL |
| 2.5 mM | 0.84 mL | 4.21 mL | 8.42 mL |
| 5 mM | 0.42 mL | 2.10 mL | 4.21 mL |
| 25 mM | 0.08 mL | 0.42 mL | 0.84 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Yang Regulation of ferroptotic cancer cell death by GPX4. Cell 2014 PMID: 24439385
- Hangauer Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition. Nature 2017 PMID: 29088702
- Weïwer Development of small-molecule probes that selectively kill cells induced to express mutant RAS. Bioorg.Med.Chem.Lett. 2012 PMID: 22297109
Product Specific Notices for ML 210
For research use only
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