Metyrapone

Name: Metyrapone
Brand: Tocris Bioscience
SKU: 3292

Tocris Bioscience, part of Bio-Techne | Catalog # 3292

Citations (29)

COA / SDS

11-β hydroxylase inhibitor

Key Product Details

Description:	11-β hydroxylase inhibitor
Chemical Name:	2-Methyl-1,2-di-3-pyridinyl-1-propanone
Purity:	≥98% (HPLC)
Molecular Weight:	226.27

View Full Technical Details for Metyrapone

Technical Data
Citations

Biological Activity for Metyrapone

Metyrapone is a cytochrome P450 inhibitor. Blocks glucocorticoid synthesis via inhibition of steroid 11-β hydroxylase (CYP11B1) activity (IC₅₀ = 7.83 μM). Also inhibits CYP3A4 and cytochrome P450-mediated ω/ω-1 hydroxylase activity.

Compound Libraries for Metyrapone

Metyrapone is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Metyrapone

M. Wt	226.27
Formula	C₁₄H₁₄N₂O
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	54-36-4
PubChem ID	4174
InChi Key	FJLBFSROUSIWMA-UHFFFAOYSA-N
Smiles	O=C(C2=CN=CC=C2)C(C)(C)C1=CC=CN=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Metyrapone

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	2.26	10
	DMSO	22.63	100

Preparing Stock Solutions for Metyrapone

The following data is based on the product molecular weight 226.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.42 mL	22.10 mL	44.19 mL
5 mM	0.88 mL	4.42 mL	8.84 mL
10 mM	0.44 mL	2.21 mL	4.42 mL
50 mM	0.09 mL	0.44 mL	0.88 mL

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Reconstitution Calculator

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References for Metyrapone

References are publications that support the biological activity of the product.

Asakura and Shichi Cytochrome P450-mediated prostaglandin omega/omega-1 hydroxylase activities in porcine ciliary body epithelial cells. Exp.Eye Res. 1992 PMID: 1426070
Park Structural and dynamic basis of broad substrate specificity, catalytic mechanism and inhibition of cytochrome P450 3A4. J.Am.Chem.Soc. 2005 PMID: 16190729
Hays Structure-activity relationship study of the inhibition of adrenal cortical 11β-hydroxylase by new metyr. analogues. J.Med.Chem. 1984 PMID: 6606707