L-trans-2,4-PDC

Name: L- trans -2,4-PDC
Brand: Tocris Bioscience
SKU: 0298

Tocris Bioscience, part of Bio-Techne | Catalog # 0298

Citations (8)

COA / SDS

Transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor

Key Product Details

Description:	Transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor
Alternative Names:	trans-4-Carboxy-L-proline
Chemical Name:	L-trans-Pyrrolidine-2,4-dicarboxylic acid
Purity:	≥97% (HPLC)
Molecular Weight:	159.14

View Full Technical Details for L-trans-2,4-PDC

Technical Data
Citations

Biological Activity for L-trans-2,4-PDC

L-trans-2,4-PDC is a potent, competitive, transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor. In [³H]-d-Asp uptake assays in HEK293 cells expressing human EAAT1, EAAT2 and EAAT3, K_i values are 20, 20 and 109 μM, respectively. In a FLIPR Membrane Potential (FMP) assay, K_m values for L-trans-2,4-PDC are 7.7, 11 and 19 μM for human EAAT2, EAAT3 and EAAT1, respectively.

Technical Data for L-trans-2,4-PDC

M. Wt	159.14
Formula	C₆H₉NO₄
Storage	Store at RT
Purity	≥97% (HPLC)
CAS Number	64769-66-0
PubChem ID	1515192
InChi Key	NRSBQSJHFYZIPH-DMTCNVIQSA-N
Smiles	O=[C@]([C@H]1NC[C@H]([C@@](O)=O)C1)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for L-trans-2,4-PDC

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	15.91	100

Preparing Stock Solutions for L-trans-2,4-PDC

The following data is based on the product molecular weight 159.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.28 mL	31.42 mL	62.84 mL
5 mM	1.26 mL	6.28 mL	12.57 mL
10 mM	0.63 mL	3.14 mL	6.28 mL
50 mM	0.13 mL	0.63 mL	1.26 mL

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References for L-trans-2,4-PDC

References are publications that support the biological activity of the product.

Jensen and Bräuner-Osborne Pharmacological characterization of human excitatory amino acid transporters EAAT1, EAAT2 and EAAT3 in a fluorescence-based membrane potential assay. Biochem.Pharmacol. 2004 PMID: 15135308
Mitrovic and Johnston Regional differences in the inhibition of L-glutamate amd L-aspartate sodium-dependent high affinity uptake systems in rat CNS synaptosomes by L-trans-pyrrolidine-2,4-dicarboxylic threo-3-hydroxy-D-aspartate and D-aspartate. Neurochem.Int. 1994 PMID: 7981641
Zuiderwijk Effects of uptake carrier blockers SK & F 89976-A and L-trans-PDC on in vivo release of amino acids in rat hippocampus. Eur.J.Pharmacol. 1996 PMID: 8836615
Bridges Conformationally defined neurotransmitter analogues. Selective inhibition of glutamate uptake by one pyrrolidine-2,4-dicarboxylate diastereomer. J.Med.Chem. 1991 PMID: 1671706