KH 7

Name: KH 7
Brand: Tocris Bioscience
SKU: 3834
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3834

Citations (9)

(1)

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Key Product Details

Description:	Selective soluble adenylyl cyclase inhibitor
Chemical Name:	(±)-2-(1H-benzimidazol-2-ylthio)propanoic acid 2-[(5-bromo-2-hydroxyphenyl)methylene]hydrazide
Purity:	≥99% (HPLC)
Molecular Weight:	419.3

View Full Technical Details for KH 7

Biological Activity for KH 7

KH 7 is a selective soluble adenylyl cyclase (sAC) inhibitor (IC₅₀ = 3 - 10 μM in vivo). Inert towards transmembrane adenylyl cyclase (tmAC) in vitro and in whole cells at concentrations up to 300 μM. Blocks synthesis of cAMP and displays an antiapoptotic effect at concentrations of 1 - 100 μM.

Technical Data for KH 7

M. Wt	419.3
Formula	C₁₇H₁₅BrN₄O₂S
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	330676-02-3
PubChem ID	6843191
InChi Key	GEHVZUUHMWNMAY-UHFFFAOYSA-N
Smiles	OC(C=CC(Br)=C2)=C2/C=N/NC(C(C)SC1=NC(C=CC=C3)=C3N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for KH 7

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	41.93	100

Preparing Stock Solutions for KH 7

The following data is based on the product molecular weight 419.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.38 mL	11.92 mL	23.85 mL
5 mM	0.48 mL	2.38 mL	4.77 mL
10 mM	0.24 mL	1.19 mL	2.38 mL
50 mM	0.05 mL	0.24 mL	0.48 mL

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References for KH 7

References are publications that support the biological activity of the product.

Kumar Soluble adenylyl cyclase controls mitochondria-dependent apoptosis in coronary endothelial cells. J.Biol.Chem. 2009 PMID: 19336406
Stessin Soluble adenylyl cyclase mediates nerve growth factor-induced activation of Rap1. J.Biol.Chem. 2006 PMID: 16627466
Hess The 'soluble' adenylyl cyclase in sperm mediates multiple signaling events required for fertilization. Develop.Cell

Citations for KH 7 (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for KH 7 include:

Schnúr et al. (2019) Phosphorylation-dependent modulation of CFTR macromolecular signalling complex activity by cigarette smoke condensate in airway epithelia. Sci Rep. 9:12706
PMID: 31481727
Ishii et al. (2019) An improved method for isolation of mitochondria from cell lines that enables reconstitution of calcium-dependent processes. Anal Biochem. 577:52
PMID: 31022379
Yamada et al. (2018) Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation. J Reprod Dev. 64:377
PMID: 29952339
Inda et al. (2017) cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells. Sci Rep. 7:1944
PMID: 28512295
Inda et al. (2016) Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling. J Cell Biol. 214:181
PMID: 27402953
Shahidullah et al. (2014) Nonpigmented ciliary epithelial cells respond to acetazol. by a soluble adenylyl cyclase mechanism. Invest Ophthalmol Vis Sci. 55:187
PMID: 24282227
Jahchan et al. (2013) A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors. Cancer Discov. 3:1364
PMID: 24078773
Chen et al. (2013) Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway. Mediators Inflamm. 9:2033
PMID: 24145604
Lee et al. (2012) The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca²⁺ and cAMP. Nature. 492:123
PMID: 23143333

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a selective sAC inhibitor

Name: Hyun Choi

Species: Rat

Assay Type: Ex Vivo

Used KH7 to block soluble adenylyl cyclase which is sensitive to bicarbonate. Used at 20uM in rat brain slice.

pretreat KH7 at least 30 min before treatment

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Product Documents for KH 7

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.