JNJ 47965567

Name: JNJ 47965567
Brand: Tocris Bioscience
SKU: 5299
Rating: 4 (3 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 5299

Citations (2)

(3)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Potent and selective P2X₇ antagonist; brain penetrant
Chemical Name:	2-(Phenylthio)-N-[[tetrahydro-4-(4-phenyl-1-piperazinyl)-2H-pyran-4-yl]methyl-3-pyridinecarboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	488.64

View Full Technical Details for JNJ 47965567

Biological Activity for JNJ 47965567

JNJ 47965567 is a potent and selective P2X₇ antagonist (pIC₅₀ values are 8.3, 7.5 and 7.2 for human, mouse and rat receptors respectively). Selective over a panel of 50 other receptors, ion channels and transporters. Reduces BzATP-induced IL-1β release from monocytes in vitro and from rat brain in vivo. Attenuates amphetamine-induced hyperactivity in rats. Brain penetrant.

Compound Libraries for JNJ 47965567

JNJ 47965567 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for JNJ 47965567

M. Wt	488.64
Formula	C₂₈H₃₂N₄O₂S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1428327-31-4
PubChem ID	66553218
InChi Key	XREFXUCWSYMIOG-UHFFFAOYSA-N
Smiles	O=C(NCC4(CCOCC4)N3CCN(C5=CC=CC=C5)CC3)C1=CC=CN=C1SC2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JNJ 47965567

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. HCl	24.43	50
	DMSO	48.86	100

Preparing Stock Solutions for JNJ 47965567

The following data is based on the product molecular weight 488.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.05 mL	10.23 mL	20.46 mL
5 mM	0.41 mL	2.05 mL	4.09 mL
10 mM	0.20 mL	1.02 mL	2.05 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for JNJ 47965567

References are publications that support the biological activity of the product.

Bhattacharya Pharmacological characterization of a novel centrally permeable P2X₇ receptor antagonist: JNJ-47965567. Br.J.Pharmacol. 2013 PMID: 23889535
Letavic Synthesis and pharmacological characterization of two novel, brain penetrating P2X₇ antagonists. ACS Med.Chem.Lett. 2013 PMID: 24900687

Citations for JNJ 47965567 (2)

Showing 1 - 2 of 2 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for JNJ 47965567 include:

McEwan et al. (2021) Autocrine regulation of wound healing by ATP release and P2Y 2 receptor activation Life Sci. 283:119850
PMID: 34314735
Uderhardt et al. (2019) Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell. 177:541
PMID: 30955887

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Customer Reviews for JNJ 47965567 (3)

4.3 out of 5

3 customer ratings

5 stars

67%

4 stars

3 stars

33%

2 stars

1 star

Customer Images

Showing 1 - 3 of 3 reviews Showing All

Filter By:

Good inhibitor

Name: Stefan Uderhardt

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: phagocyte

DMSO dilution 100 μM

PMID: PMID: 30955887
In vivo usage

Name: Spandita Dutta

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: Hippocampal neurons

I have used this product emulsified in 30% sulphoxymethylester, and injected them sub-cutaneously to study IL-1beta release mechanism in the hippocampus of the brain. I am currently working on its effect on hippocampal neurons in culture.

Followed publication by Bhattacharya et al, Br J Pharmacol. 2013 Oct;170(3):624-40. doi: 10.1111/bph.12314 for in vivo and in vitro usage.
P2X7 blocker

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Used to block a potentially P2X7-dependent process, but no effect was observed. No positive control so unsure if the drug actually blocked its target. More experiments needed.

There are no reviews that match your criteria.

Showing 1 - 3 of 3 reviews Showing All

Product Documents for JNJ 47965567

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.