JH 295
Tocris Bioscience, part of Bio-Techne | Catalog # 4322
Irreversible Nek2 inhibitor
Product Description
JH 295 is an irreversible Nek2 inhibitor (IC50=770 nM). Inhibits Nek2 via alkylation of residue Cys22. Displays 5-fold selectivity for Nek2 over Cdk1. Exhibits no significant effect on Aurora B or Plk1 kinases, bipolar spindle assembly or spindle assembly checkpoint. Cell permeable.Product Specifications
Molecular Weight
320.35
Formula
C18H16N4O2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
N-[(3Z)-3-[(2-Ethyl-4-methyl-1H-imidazol-5-yl)methylene]-2,3-dihydro-2-oxo-1H-indol-5-yl]-2-propynamide
CAS Number
1311143-71-1
PubChem ID
53262909
InChI Key
ORKOHXAJFGRZCL-LCYFTJDESA-N
SMILES
O=C2NC1=CC=C(NC(C#C)=O)C=C1/C2=C/C3=C(C)N=C(CC)N3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.03 | 100 |
Preparing Stock Solutions
for JH 295
The following data is based on the product molecular weight 320.35
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.22 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.24 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Henise Irreversible Nek2 kinase inhibitors with cellular activity. J.Med.Chem. 2011 PMID: 21627121
Product Specific Notices for JH 295
For research use only
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