iCRT 14
Tocris Bioscience, part of Bio-Techne | Catalog # 4299
Inhibits β-catenin-responsive transcription (CRT)
Key Product Details
Description
Inhibits β-catenin-responsive transcription (CRT)
Product Description
iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.Product Specifications
Molecular Weight
375.44
Formula
C21H17N3O2S
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion
CAS Number
677331-12-3
PubChem ID
5967294
InChI Key
NCSHZXNGQYSKLR-UNOMPAQXSA-N
SMILES
CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 28.16 | 75 |
Preparing Stock Solutions
for iCRT 14
The following data is based on the product molecular weight 375.44
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.75 mM | 3.55 mL | 17.76 mL | 35.51 mL |
| 3.75 mM | 0.71 mL | 3.55 mL | 7.10 mL |
| 7.5 mM | 0.36 mL | 1.78 mL | 3.55 mL |
| 37.5 mM | 0.07 mL | 0.36 mL | 0.71 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Watanabe and Dai Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 2011 PMID: 21451134
- Spivack Enhancement of transgene expression by the β-catenin inhibitor iCRT14. Plasmid 2021 PMID: 33472046
- Gonsalves An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 2011 PMID: 21393571
Product Specific Notices for iCRT 14
For research use only
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