iCRT 14

Name: iCRT 14
Brand: Tocris Bioscience
SKU: 4299
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4299

(1)

Citations (37)

COA / SDS

Inhibits β-catenin-responsive transcription (CRT)

Key Product Details

Description:	Inhibits β-catenin-responsive transcription (CRT)
Chemical Name:	5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion
Purity:	≥98% (HPLC)
Molecular Weight:	375.44

View Full Technical Details for iCRT 14

Technical Data
Citations / Reviews (1)

Biological Activity for iCRT 14

iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC₅₀ = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G₀/G₁ cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.

Compound Libraries for iCRT 14

iCRT 14 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for iCRT 14

M. Wt	375.44
Formula	C₂₁H₁₇N₃O₂S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	677331-12-3
PubChem ID	5967294
InChi Key	NCSHZXNGQYSKLR-UNOMPAQXSA-N
Smiles	CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for iCRT 14

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	28.16	75

Preparing Stock Solutions for iCRT 14

The following data is based on the product molecular weight 375.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.75 mM	3.55 mL	17.76 mL	35.51 mL
3.75 mM	0.71 mL	3.55 mL	7.10 mL
7.5 mM	0.36 mL	1.78 mL	3.55 mL
37.5 mM	0.07 mL	0.36 mL	0.71 mL

Molarity Calculator

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Reconstitution Calculator

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References for iCRT 14

References are publications that support the biological activity of the product.

Watanabe and Dai Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 2011 PMID: 21451134
Spivack Enhancement of transgene expression by the β-catenin inhibitor iCRT14. Plasmid 2021 PMID: 33472046
Gonsalves An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 2011 PMID: 21393571