iBET-BD2

Name: iBET-BD2
Brand: Tocris Bioscience
SKU: 7458

Tocris Bioscience, part of Bio-Techne | Catalog # 7458

COA / SDS

Potent and selective pan-BD2 inhibitor; orally bioavailable

Key Product Details

Description:	Potent and selective pan-BD2 inhibitor; orally bioavailable
Alternative Names:	GSK046
Chemical Name:	4-(Acetylamino)-3-fluoro-N-(trans-4-hydroxycyclohexyl)-5-[(1S)-1-phenylethoxy]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	414.48

View Full Technical Details for iBET-BD2

Technical Data
Citations

Biological Activity for iBET-BD2

iBET-BD2 is a potent and selective pan-BD2 inhibitor (IC₅₀ values are 49, 98, 214 and 264 nM for BRD4BD2, BRD3BD2, BRDTBD2 and BRD2BD2, respectively). iBET-BD2 is selective for BD2 over BD1 (IC₅₀ values are 10965, 36317, 70558, and >50119 µM for BRD2BD1, BRD3BD1, BRD4BD1, and BRDTBD1, respectively) in a time-resolved FRET (TR-FRET) assay. iBET-BD2 (1 μM) inhibits IFN-γ-induced protein expression of MHC class I in K562 cells. iBET-BD2 reduces the production of anti-KLH IgM antibodies in mice immunized with keyhole limpet hemocyanin (KLH). iBET-BD2 exhibits immunomodulatory activity. iBET-BD2 is orally bioavailable.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the iBET-BD2 probe summary on the SGC website.

Compound Libraries for iBET-BD2

iBET-BD2 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for iBET-BD2

M. Wt	414.48
Formula	C₂₃H₂₇FN₂O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2474876-09-8
PubChem ID	146018690
InChi Key	FRBRZGLUFOZRGD-JVPBZIDWSA-N
Smiles	C[C@@H](C1=CC=CC=C1)OC2=C(C(F)=CC(C(N[C@H]3CC[C@@H](CC3)O)=O)=C2)NC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for iBET-BD2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	41.45	100
	ethanol	41.45	100

Preparing Stock Solutions for iBET-BD2

The following data is based on the product molecular weight 414.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.41 mL	12.06 mL	24.13 mL
5 mM	0.48 mL	2.41 mL	4.83 mL
10 mM	0.24 mL	1.21 mL	2.41 mL
50 mM	0.05 mL	0.24 mL	0.48 mL

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Reconstitution Calculator

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References for iBET-BD2

References are publications that support the biological activity of the product.

Preston Design and synthesis of a highly selective and in vivo-capable inhibitor of the second bromodomain of the bromodomain and extra terminal domain family of proteins. J.Med.Chem. 2020 PMID: 32691591
Gilan Selective targeting of BD1 and BD2 of the BET proteins in cancer and immunoinflammation. Science 2020 PMID: 32193360