Hh-Ag1.5
Tocris Bioscience, part of Bio-Techne | Catalog # 7807
Potent and high affinity Smo receptor agonist
Key Product Details
Description
Potent and high affinity Smo receptor agonist
Product Description
Hh-Ag1.5 is a potent and high affinity Smoothened (Smo) receptor agonist (EC50 = 1 nM; Ki values are 0.5 and 2.3 nM respectively, in a [3H]SAG-1.3 and [3H]Cyclopamine binding assay). It is used in protocols for the chemical reprogramming of mouse embryonic fibroblasts into neural stem cells. Also induces differentiation of hiPSCs into skin-derived precursor cells, spinal motor neurons and spinal sensory neurons.Product Specifications
Molecular Weight
526.04
Formula
C28H26ClF2N3OS
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
3-Chloro-4,7-difluoro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
CAS Number
612542-14-0
PubChem ID
44195701
InChI Key
HRUSRVLDSXRNFP-UPDYAAQLSA-N
SMILES
O=C(N(CC1=CC(C2=CC=NC=C2)=CC=C1)[C@H]3CC[C@H](NC)CC3)C(SC4=C(C=CC(F)=C54)F)=C5Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 52.6 | 100 | |
| Ethanol | 26.3 | 50 |
Preparing Stock Solutions
for Hh-Ag1.5
The following data is based on the product molecular weight 526.04
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.90 mL | 9.50 mL | 19.01 mL |
| 5 mM | 0.38 mL | 1.90 mL | 3.80 mL |
| 10 mM | 0.19 mL | 0.95 mL | 1.90 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.38 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Sugiyama-Nakagiri Induction of skin-derived precursor cells from human induced pluripotent stem cells. PLoS One 2016 PMID: 27992514
- Rominger Evidence for allosteric interactions of antagonist binding to the smoothened receptor. J.Pharmacol.Exp.Ther. 2009 PMID: 19304771
- Takeda Chemical compound-based direct reprogramming for future clinical applications. Biosci.Rep. 2018 PMID: 29739872
- Frank-Kamenetsky Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. J.Biol. 2002 PMID: 12437772
Product Specific Notices for Hh-Ag1.5
For research use only
Loading...
Loading...