GSK J4
Tocris Bioscience, part of Bio-Techne | Catalog # 4594
Histone KDM inhibitor; cell permeable
Key Product Details
Description
Histone KDM inhibitor; cell permeable
Product Description
GSK J4 is a histone lysine demethylase (KDM) inhibitor; blocks demethylation of histone H3K27. Attenuates lipopolysaccharide (LPS)-induced proinflammatory cytokine production in primary human macrophages (IC50 = 9 μM for the inhibition of TNFα release). Cell permeable. Ethyl ester derivative of GSK J1.Negative Control also available.
Licensing Information
This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details of GSK J4, a cell permeable derivative of GSK J1, please visit the GSK J1 probe summary on the SGC website.External Portal Information
Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of GSK J4 is reviewed on the chemical probes website.Product Specifications
Molecular Weight
417.50
Formula
C24H27N5O2
Storage
Store at RT
Purity
≥98% (HPLC)
Chemical Name
N-[2-(2-Pyridinyl)-6-(1,2,4,5-tetrahydro-3H-3-benzazepin-3-yl)-4-pyrimidinyl]-β-alanine ethyl ester
CAS Number
1373423-53-0
PubChem ID
71729975
InChI Key
WBKCKEHGXNWYMO-UHFFFAOYSA-N
SMILES
O=C(CCNC1=NC(C2=CC=CC=N2)=NC(N3CCC(C=CC=C4)=C4CC3)=C1)OCC
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.75 | 100 | |
| Ethanol | 41.75 | 100 |
Preparing Stock Solutions
for GSK J4
The following data is based on the product molecular weight 417.50
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.40 mL | 11.98 mL | 23.95 mL |
| 5 mM | 0.48 mL | 2.40 mL | 4.79 mL |
| 10 mM | 0.24 mL | 1.20 mL | 2.40 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Heinemann Inhibition of demethylases by GSK-J1/J4. Nature 2014 PMID: 25279926
- Kruidenier A selective jumonji H3K27 demethylase inhibitor modulates the proinflammatory macrophage response. Nature 2012 PMID: 22842901
Product Specific Notices for GSK J4
For research use only
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