GRL 0617

Name: GRL 0617
Brand: Tocris Bioscience
SKU: 7280

Tocris Bioscience, part of Bio-Techne | Catalog # 7280

Citations (2)

COA / SDS

Coronavirus PLpro inhibitor

Key Product Details

Description:	Coronavirus PLpro inhibitor
Chemical Name:	5-Amino-2-methyl-N-[(1R)-1-(1-naphthalenyl)ethyl]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	304.39

View Full Technical Details for GRL 0617

Technical Data
Citations

Biological Activity for GRL 0617

GRL 0617 is a coronavirus papain-like protease (Plpro) inhibitor (IC₅₀ values are 0.6 and 0.8 μM for SARS-COV and SARS-CoV-2 PLpro, respectively). Inhibits deubiquitination activity of SARS-CoV PLpro. Displays no inhibition of human deubiquitinating enzymes. Improves cell viability following SARS-CoV infection. Inhibits SARS-CoV-2 viral replication and induces antiviral immune signaling pathways following SARS-CoV-2 infection in CaCo-2 cells.

Compound Libraries for GRL 0617

GRL 0617 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for GRL 0617

M. Wt	304.39
Formula	C₂₀H₂₀N₂O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1093070-16-6
PubChem ID	24941262
InChi Key	UVERBUNNCOKGNZ-CQSZACIVSA-N
Smiles	C[C@H](C1=CC=CC2=C1C=CC=C2)NC(C3=C(C=CC(N)=C3)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GRL 0617

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	30.44	100
	ethanol	30.44	100

Preparing Stock Solutions for GRL 0617

The following data is based on the product molecular weight 304.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.29 mL	16.43 mL	32.85 mL
5 mM	0.66 mL	3.29 mL	6.57 mL
10 mM	0.33 mL	1.64 mL	3.29 mL
50 mM	0.07 mL	0.33 mL	0.66 mL

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Reconstitution Calculator

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References for GRL 0617

References are publications that support the biological activity of the product.

Freitas Characterization and noncovalent inhibition of the deubiquitinase and deISGylase activity of SARS-CoV-2 papain-Like protease. ACS Infect.Dis. 2020 PMID: 32428392
Shin Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 PMID: 32726803
Ratia A noncovalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication. Proc.Natl.Acad.Sci.USA. 2008 PMID: 18852458