GF 109203X

Name: GF 109203X
Brand: Tocris Bioscience
SKU: 0741
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 0741

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Citations (302)

COA / SDS

Protein kinase C inhibitor

Key Product Details

Description:	Protein kinase C inhibitor
Alternative Names:	Gö 6850,Bisindolylmaleimide I
Chemical Name:	2-[1-(3-Dimethylaminopropyl)indol-3-yl]-3-(indol-3-yl) maleimide
Purity:	≥98% (HPLC)
Molecular Weight:	412.49

View Full Technical Details for GF 109203X

Technical Data
Citations / Reviews (1)

Biological Activity for GF 109203X

GF 109203X is a very potent and selective inhibitor of protein kinase C, selective for the α and β1 isoforms (IC₅₀ values are 0.0084, 0.0180, 0.210, 0.132, and 5.8 μM for α, β1, δ, ε and ζ isoforms respectively). Selective over MLCK, PKG and PKA (IC₅₀ values are 0.6, 4.6, and 33 μM respectively). Potent antagonist at the 5-HT₃ receptor (K_i = 29.5 nM). Anti-inflammatory in vivo.

Compound Libraries for GF 109203X

GF 109203X is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for GF 109203X

M. Wt	412.49
Formula	C₂₅H₂₄N₄O₂
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	133052-90-1
PubChem ID	2396
InChi Key	QMGUOJYZJKLOLH-UHFFFAOYSA-N
Smiles	CN(C)CCCN1C=C(C2=CC=CC=C12)C1=C(C(=O)NC1=O)C1=CNC2=CC=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GF 109203X

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	10.31	25

Preparing Stock Solutions for GF 109203X

The following data is based on the product molecular weight 412.49.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	9.70 mL	48.49 mL	96.97 mL
1.25 mM	1.94 mL	9.70 mL	19.39 mL
2.5 mM	0.97 mL	4.85 mL	9.70 mL
12.5 mM	0.19 mL	0.97 mL	1.94 mL

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References for GF 109203X

References are publications that support the biological activity of the product.

Jiang Heat shock protein 90-mediated inactivation of nuclear factor-κB switches autophagy to apoptosis through becn1 transcriptional inhibition in selenite-induced NB4 cells. Mol.Biol.Cell. 2011 PMID: 21346199
Toullec The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J.Biol.Chem. 1991 PMID: 1874734
Jacobson Anti-inflammatory properties of Go 6850: a selective inhibitor of protein kinase C. J.Pharmacol.Exp.Ther. 1995 PMID: 7473193
Martiny-Baron Selective inhibition of protein kinase C isozymes by the indolocarbazole Go 6976. J.Biol.Chem. 1993 PMID: 8486620
Coultrap Competitive antagonism of the mouse 5-hydroxytryptamine3 receptor by bisindolylmaleimide I, a "selective" protein kinase C inhibitor. J.Pharmacol.Exp.Ther. 1999 PMID: 10381762