FCCP

Name: FCCP
Brand: Tocris Bioscience
SKU: 0453
Rating: 5 (2 reviews)

Catalog # 0453 | Tocris Bioscience a Bio-Techne Brand

(2)

Citations (12)

COA / SDS

Oxidative phosphorylation uncoupler

Key Product Details

Description:	Oxidative phosphorylation uncoupler
Chemical Name:	Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
Purity:	≥99% (HPLC)
Molecular Weight:	254.17

View Full Technical Details for FCCP

Technical Data
Citations (12) / Reviews (2)

Biological Activity for FCCP

FCCP is a very potent uncoupler of oxidative phosphorylation in mitochondria.

Technical Data for FCCP

M. Wt	254.17
Formula	C₁₀H₅F₃N₄O
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	370-86-5
PubChem ID	3330
InChi Key	BMZRVOVNUMQTIN-UHFFFAOYSA-N
Smiles	N#CC(C#N)=NNC1=CC=C(OC(F)(F)F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FCCP

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	25.42	100

Preparing Stock Solutions for FCCP

The following data is based on the product molecular weight 254.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.93 mL	19.67 mL	39.34 mL
5 mM	0.79 mL	3.93 mL	7.87 mL
10 mM	0.39 mL	1.97 mL	3.93 mL
50 mM	0.08 mL	0.39 mL	0.79 mL

Molarity Calculator

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Reconstitution Calculator

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References for FCCP

References are publications that support the biological activity of the product.

Connop Novel effects of FCCP [carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone] on amyloid precursor protein processing. J.Neurochem. 1999 PMID: 10098849
Heytler and Pritchard A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Commun. 1962 PMID: 13907155

Citations for FCCP (12)

Showing 1 - 10 of 12 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for FCCP include:

MacDonald et al. (2019) A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics. Commun Biol. 2:258
PMID: 31312727
Panja et al. (2019) Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity. Neuropathol Appl Neurobiol. 45:141
PMID: 29679372
MacDonald et al. (2018) Mitochondrial membrane depolarization enhances TRAIL-induced cell death in adult human granulosa tumor cells, KGN, through inhibition of BIRC5. J Ovarian Res. 11:89
PMID: 30326924
Rozzi et al. (2018) Human immunodeficiency virus Tat impairs mitochondrial fission in neurons. Cell Death Discov. 4:8
PMID: 29531805
Visavadiya (2016) Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3. Cell Communication and Signaling. 14:32
PMID: 27978828
Banh et al. (2016) PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia. Nat Cell Biol. 18:803
PMID: 27323329
Choubey et al. (2014) BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy. 10:1105
PMID: 24879156
Janc (2014) The free radical scavenger Trolox dampens neuronal hyperexcitability, reinstates synaptic plasticity, and improves hypoxia tolerance in a mouse model of Rett syndrome. Front Cell Neurosci. 8:56
PMID: 24605086
Hogan et al. (2014) Divergent effects of painful nerve injury on mitochondrial Ca(2+) buffering in axotomized and adjacent sensory neurons. Brain Res. 1589:112
PMID: 25251590
Burt et al. (2013) 9-Phenanthrol and flufenamic acid inhibit calcium oscillations in HL-1 mouse cardiomyocytes. Cell Calcium. 54:193
PMID: 23831210
Man&eacute and Müller (2012) Temporo-spectral imaging of intrinsic optical signals during hypoxia-induced spreading depression-like depolarization. PLoS One. 7:e43981
PMID: 22952835
Gerich et al. (2009) H(2)O(2)-mediated modulation of cytosolic signaling and organelle function in rat hippocampus. J Pharmacol Exp Ther. 458:937
PMID: 19430810

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Showing 1 - 10 of 12 citations Showing All

Customer Reviews for FCCP (2)

4.5 out of 5

2 customer ratings

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We got expected effect on cells

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Primary pancreatic ductal epithelial cells

4 μM FCCP

PMID: PMID: 27799657
Mitochondrial bioenergetics measurements by reagent screen

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: mouse brain endothelioma cell line

1 μM

PMID: 27978828

There are no reviews that match your criteria.