Evans Blue tetrasodium salt

Name: Evans Blue tetrasodium salt
Brand: Tocris Bioscience
SKU: 0845
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 0845

Citations (2)

(1)

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Key Product Details

Description:	Dye for assessing cell viability and blood brain barrier permeability; also EAAT inhibitor and iGluR antagonist
Chemical Name:	6,6-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)bis[4-amino- 5-hydroxy-1,3-naphthalenedisulphonic acid] tetrasodium salt
Purity:	≥90% (HPLC)
Molecular Weight:	960.82

View Full Technical Details for Evans Blue tetrasodium salt

Biological Activity for Evans Blue tetrasodium salt

Evans Blue tetrasodium salt is a potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC₅₀ values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist. Also a non-permeating dye, stains damaged plasma membrane and enters in the cytoplasm and nucleus, used for checking cell viability.

Optical Data for Evans Blue tetrasodium salt

λ_em	680 nm

Technical Data for Evans Blue tetrasodium salt

M. Wt	960.82
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Storage	Store at RT
Purity	≥90% (HPLC)
CAS Number	314-13-6
PubChem ID	101102794
InChi Key	ATNOAWAQFYGAOY-UHFFFAOYSA-J
Smiles	[Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1\N=N\C1=C(O)C2=C(C=C1)C(=CC(=C2N)S([O-])(=O)=O)S([O-])(=O)=O)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S([O-])(=O)=O)S([O-])(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Evans Blue tetrasodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	9.61	10

Preparing Stock Solutions for Evans Blue tetrasodium salt

The following data is based on the product molecular weight 960.82.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	10.41 mL	52.04 mL	104.08 mL
0.5 mM	2.08 mL	10.41 mL	20.82 mL
1 mM	1.04 mL	5.20 mL	10.41 mL
5 mM	0.21 mL	1.04 mL	2.08 mL

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References for Evans Blue tetrasodium salt

References are publications that support the biological activity of the product.

Schurmann Differential modulation of AMPA receptor mediated currents by Evans Blue in postnatal rat hippocampal neurones. Br.J.Pharmacol. 1997 PMID: 9154333
Price and Raymond Evans blue antagonizes both α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates desensitization. Mol. Pharmacol. 1996 PMID: 8967991
Wittenburg P₂-purinoceptor antagonists:II blockade of P₂-purinoceptor subtypes and ecto-nucleotidase by compounds related to Evans blue and trypan blue. Naunyn Schmiedebergs Arch.Pharmacol. 1996 PMID: 8897453
Roseth Uptake of L-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. J.Neurochem. 1995 PMID: 7790899

Citations for Evans Blue tetrasodium salt (2)

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Citations are publications that use Bio-Techne products. Selected citations for Evans Blue tetrasodium salt include:

Uderhardt et al. (2019) Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell. 177:541
PMID: 30955887
Gao et al. (2018) Research articleHemolysis in human erythrocytes by Clostridium perfringens epsilon toxin requires activation of P2 receptors. Virulence. 9:1601
PMID: 30277122

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Used for injection

Name: Anonymous

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: muscle

Evans blue solution 50 μl/g body weight injection to allow imaging of necrotic tissue in muscle dystrophy mouse model

PMID: PMID: 30955887

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