Eeyarestatin I

Name: Eeyarestatin I
Brand: Tocris Bioscience
SKU: 3922

Tocris Bioscience a Bio-Techne Brand

Catalog # 3922

Citations (4)

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Key Product Details

Description:	Potent inhibitor of ER-associated protein degradation and translocation
Chemical Name:	3-(4-Chlorophenyl)-4-[[[(4-chlorophenyl)amino]carbonyl]hydroxyamino]-5,5-dimethyl-2-oxo-1-imidazolidineacetic acid 2-[3-(5-nitro-2-furanyl)-2-propen-1-ylidene]hydrazide
Purity:	≥98% (HPLC)
Molecular Weight:	630.44

View Full Technical Details for Eeyarestatin I

Biological Activity for Eeyarestatin I

Eeyarestatin I is a potent inhibitor of endoplasmic reticulum associated protein degradation (ERAD). Specifically targets the p97-associated deubiquitinating process (PAD) and inhibits ataxin-3 (atx3)-dependent deubiquitination. Also inhibits Sec61-mediated protein translocation at the ER. Displays cytotoxic activity preferentially against cancer cells; induces cell death via the proapoptotic protein NOXA. Also enhances adeno-associated viral transduction in multiple cells types.

Technical Data for Eeyarestatin I

M. Wt	630.44
Formula	C₂₇H₂₅Cl₂N₇O₇
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	412960-54-4
PubChem ID	23947580
InChi Key	JTUXTPWYZXWOIB-LWWHHIEBSA-N
Smiles	O=C1N(C2=CC=C(Cl)C=C2)C(N(O)C(NC4=CC=C(Cl)C=C4)=O)C(C)(C)N1CC(N/N=C/C=C/C3=CC=C([N+]([O-])=O)O3)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Eeyarestatin I

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	63.04	100

Preparing Stock Solutions for Eeyarestatin I

The following data is based on the product molecular weight 630.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.59 mL	7.93 mL	15.86 mL
5 mM	0.32 mL	1.59 mL	3.17 mL
10 mM	0.16 mL	0.79 mL	1.59 mL
50 mM	0.03 mL	0.16 mL	0.32 mL

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References for Eeyarestatin I

References are publications that support the biological activity of the product.

Berry and Asokan Chemical modulation of endocytic sorting augments adeno-associated viral transduction. J.Biol.Chem. 2016 PMID: 26527686
Aletrari Eeyarestatin 1 interferes with both retrograde and anterograde intracellular trafficking pathways. PLoS One 2011 PMID: 21799938
Cross Eeyarestatin I inhibits Sec61-mediated protein translocation at the endoplasmic reticulum. J.Cell Science
Wang Inhibition of p97-dependent protein degradation by eeyarestatin I. J.Biol.Chem. 2008 PMID: 18199748
Wang ERAD inhibitors integrate ER stress with an epigenetic mechanism to activate BH3-only protein NOXA in cancer cells. Proc.Natl.Acad.Sci.USA

Citations for Eeyarestatin I (4)

Showing 1 - 4 of 4 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Eeyarestatin I include:

Eales et al. (2018) Verteporfin selectively kills hypoxic glioma cells through iron-binding and increased production of reactive oxygen species. Sci Rep. 8(1):14358
PMID: 30254296
Yi et al. (2016) Affinity Purification of the Hepatitis C Virus Replicase Identifies Valosin-Containing Protein, a Member of the ATPases Associated with Diverse Cellular Activities Family, as an Active Virus Replication Modulator. J Virol. 90:9953
PMID: 27558430
Han et al. (2015) Combining valosin-containing protein (VCP) inhibition and suberanilohydroxamic acid (SAHA) treatment additively enhances the folding, trafficking, and function of epilepsy-associated γ-aminobutyric acid, type A (GABAA) receptors. Cell Rep. 290:325
PMID: 25406314
Davis et al. (2015) Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins. Biochem Biophys Res Commun. 11:1727
PMID: 26074080

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Showing 1 - 4 of 4 citations Showing All

Product Documents for Eeyarestatin I

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.