Eeyarestatin I
Tocris Bioscience, part of Bio-Techne | Catalog # 3922
Potent inhibitor of ER-associated protein degradation and translocation
Key Product Details
Description
Potent inhibitor of ER-associated protein degradation and translocation
Product Description
Eeyarestatin I is a potent inhibitor of endoplasmic reticulum associated protein degradation (ERAD). Specifically targets the p97-associated deubiquitinating process (PAD) and inhibits ataxin-3 (atx3)-dependent deubiquitination. Also inhibits Sec61-mediated protein translocation at the ER. Displays cytotoxic activity preferentially against cancer cells; induces cell death via the proapoptotic protein NOXA. Also enhances adeno-associated viral transduction in multiple cells types.Product Specifications
Molecular Weight
630.44
Formula
C27H25Cl2N7O7
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
3-(4-Chlorophenyl)-4-[[[(4-chlorophenyl)amino]carbonyl]hydroxyamino]-5,5-dimethyl-2-oxo-1-imidazolidineacetic acid 2-[3-(5-nitro-2-furanyl)-2-propen-1-ylidene]hydrazide
CAS Number
412960-54-4
PubChem ID
23947580
InChI Key
JTUXTPWYZXWOIB-LWWHHIEBSA-N
SMILES
O=C1N(C2=CC=C(Cl)C=C2)C(N(O)C(NC4=CC=C(Cl)C=C4)=O)C(C)(C)N1CC(N/N=C/C=C/C3=CC=C([N+]([O-])=O)O3)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 63.04 | 100 |
Preparing Stock Solutions
for Eeyarestatin I
The following data is based on the product molecular weight 630.44
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.59 mL | 7.93 mL | 15.86 mL |
| 5 mM | 0.32 mL | 1.59 mL | 3.17 mL |
| 10 mM | 0.16 mL | 0.79 mL | 1.59 mL |
| 50 mM | 0.03 mL | 0.16 mL | 0.32 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Berry and Asokan Chemical modulation of endocytic sorting augments adeno-associated viral transduction. J.Biol.Chem. 2016 PMID: 26527686
- Cross Eeyarestatin I inhibits Sec61-mediated protein translocation at the endoplasmic reticulum. J.Cell Science
- Aletrari Eeyarestatin 1 interferes with both retrograde and anterograde intracellular trafficking pathways. PLoS One 2011 PMID: 21799938
- Wang ERAD inhibitors integrate ER stress with an epigenetic mechanism to activate BH3-only protein NOXA in cancer cells. Proc.Natl.Acad.Sci.USA
- Wang Inhibition of p97-dependent protein degradation by eeyarestatin I. J.Biol.Chem. 2008 PMID: 18199748
Product Specific Notices for Eeyarestatin I
For research use only
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