dTAG-Fluorescein
Tocris Bioscience, part of Bio-Techne | Catalog # 7892
Fluorescent probe for labeling mutant FKBP12 (F36V) proteins
Key Product Details
Description
Fluorescent probe for labeling mutant FKBP12 (F36V) proteins
Product Description
dTAG-Fluorescein is a fluorescent probe for labeling FKBP12F36V fusion proteins. Comprises a high-affinity ligand for FKBP12F36V mutant protein (Kd in the subnanomolar range and 1,000-fold selectivity for the F36V mutant of FKBP12 over wild-type) coupled to 5-amino fluorescein. dTAG-Fluorescein can fluorescently label FKBP12F36V fusion proteins expressed at different levels in a variety of cell lines.Optical Data
| Emission Color | Green |
| λabs | 493 nm |
| λem | 514 nm |
| Extinction Coefficient (ε) | 68800 M-1cm-1 |
| Closest Laser line | 488 nm |
| Application | Confocal microscopy |
Product Specifications
Molecular Weight
1023.10
Formula
C58H58N2O15
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(R)-1-(2-(2-((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)amino)-2-oxoethoxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
InChI Key
ZSRZNOZTAUPBBF-ZSDGZGBPSA-N
SMILES
CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NC4=CC(C(O5)=O)=C(C=C4)C65C7=C(C=C(C=C7)O)OC8=C6C=CC(O)=C8)=O)CCC9=CC(OC)=C(C=C9)OC)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 10.23 | 10 |
Preparing Stock Solutions
for dTAG-Fluorescein
The following data is based on the product molecular weight 1023.10
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 9.77 mL | 48.87 mL | 97.74 mL |
| 0.5 mM | 1.95 mL | 9.77 mL | 19.55 mL |
| 1 mM | 0.98 mL | 4.89 mL | 9.77 mL |
| 5 mM | 0.20 mL | 0.98 mL | 1.95 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Marks A general approach for chemical labeling and rapid, spatially controlled protein inactivation. Proc.Natl.Acad.Sci.U.S.A. 2004 PMID: 15218100
- Nabet The dTAG system for immediate and target-specific protein degradation. Nat.Chem.Biol. 2018 PMID: 29581585
Product Specific Notices for dTAG-Fluorescein
For research use only
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