DPTIP

Name: DPTIP
Brand: Tocris Bioscience
SKU: 6903

Tocris Bioscience, part of Bio-Techne | Catalog # 6903

COA / SDS

Potent and selective N-SMase2 inhibitor; brain penetrant

Key Product Details

Description:	Potent and selective N-SMase2 inhibitor; brain penetrant
Chemical Name:	2,6-Dimethoxy-4-[4-phenyl-5-(2-thienyl)-1H-imidazol-2-yl]phenol
Purity:	≥97% (HPLC)
Molecular Weight:	378.45

View Full Technical Details for DPTIP

Technical Data
Citations

Biological Activity for DPTIP

DPTIP is a potent and selective sphingomyelin phosphodiesterase (N-SMase2) inhibitor (IC₅₀ = 30 nM). Exhibits no inhibition of related enzymes alkaline phosphatase, phosphomonoesterase or acid sphingomyelinase. Inhibits extracellular vesicle (EV) release in primary astrocyte cultures and in vivo. Attenuates IL-1β-induced astrocyte-derived EV release in a mouse model of brain injury and suppresses infiltration of immune cells into the brain. Metabolically stable and brain penetrant.

Technical Data for DPTIP

M. Wt	378.45
Formula	C₂₁H₁₈N₂O₃S
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	351353-48-5
PubChem ID	1131802
InChi Key	JMXVHYPSBANVAQ-UHFFFAOYSA-N
Smiles	COC1=CC(C2=NC(C3=CC=CC=C3)=C(C4=CC=CS4)N2)=CC(OC)=C1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DPTIP

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	37.84	100

Preparing Stock Solutions for DPTIP

The following data is based on the product molecular weight 378.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.64 mL	13.21 mL	26.42 mL
5 mM	0.53 mL	2.64 mL	5.28 mL
10 mM	0.26 mL	1.32 mL	2.64 mL
50 mM	0.05 mL	0.26 mL	0.53 mL

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References for DPTIP

References are publications that support the biological activity of the product.

Rojas DPTIP, a newly identified potent brain penetrant neutral sphingomyelinase 2 inhibitor, regulates astrocyte-peripheral immune communication following brain inflammation. Sci.Rep. 2018 PMID: 30531925