DOTAP
Tocris Bioscience, part of Bio-Techne | Catalog # 7179
Cationic agent for liposome and LNP formation
Key Product Details
Description
Cationic agent for liposome and LNP formation
Alternative Names
1,2-dioleoyl-3-trimethylammoniumpropane
Product Description
DOTAP is a cationic agent used in liposome formation. Facilitates the transfection of DNA, RNA, and other molecules with negative charges into eukaryotic cells. Can be used to generate selective organ-targeting (SORT) lipid nanoparticles.For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.
Product Specifications
Molecular Weight
698.54
Formula
C42H80ClNO4
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
N,N,N-Trimethyl-2,3-bis(oleoyloxy)propan-1-aminium chloride
CAS Number
132172-61-3
InChI Key
KSXTUUUQYQYKCR-LQDDAWAPNA-M
SMILES
C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C.[Cl-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| chloroform | 69.85 | 100 | |
| Ethanol | 69.85 | 100 |
Preparing Stock Solutions
for DOTAP
The following data is based on the product molecular weight 698.54
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.43 mL | 7.16 mL | 14.32 mL |
| 5 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 10 mM | 0.14 mL | 0.72 mL | 1.43 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.29 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Wang Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery. Nat.Protoc. 2023 PMID: 36316378
Product Specific Notices for DOTAP
For research use only
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