Donepezil hydrochloride

Name: Donepezil hydrochloride
Brand: Tocris Bioscience
SKU: 4385
Price: 89 USD

Tocris Bioscience a Bio-Techne Brand

Catalog # 4385

Citations (2)

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Key Product Details

Description:	Potent AChE inhibitor
Alternative Names:	E 2020
Chemical Name:	2,3-Dihydro-5,6-dimethoxy-2-[[1-(phenylmethyl)-4-piperidinyl]methyl]-1H-inden-1-one hydrochloride
Purity:	≥99% (HPLC)
Molecular Weight:	415.95

View Full Technical Details for Donepezil hydrochloride

Biological Activity for Donepezil hydrochloride

Donepezil hydrochloride is a potent and selective acetylcholinesterase inhibitor (IC₅₀ = 5.7 nM). In animal models of Alzheimer's disease, Donepezil reduces brain tissue Aβ protein and plaque deposition, restores normal glucose metabolism, reduces neuroinflammation and rescues cognitive dysfunction and memory deficits. The pro-cognitive effects are potentiated by combination with Mefloquine (Cat. No. 6819). In vitro Donepezil reduces LPS-induced inflammation and protects against mitochondrial membrane potential damage.

Technical Data for Donepezil hydrochloride

M. Wt	415.95
Formula	C₂₄H₂₉NO₃.HCl
Storage	Desiccate at RT
Purity	≥99% (HPLC)
CAS Number	120011-70-3
PubChem ID	5741
InChi Key	XWAIAVWHZJNZQQ-UHFFFAOYSA-N
Smiles	O=C2C(CC3CCN(CC4=CC=CC=C4)CC3)CC1=CC(OC)=C(OC)C=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Donepezil hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	20.8	50

Preparing Stock Solutions for Donepezil hydrochloride

The following data is based on the product molecular weight 415.95.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.81 mL	24.04 mL	48.08 mL
2.5 mM	0.96 mL	4.81 mL	9.62 mL
5 mM	0.48 mL	2.40 mL	4.81 mL
25 mM	0.10 mL	0.48 mL	0.96 mL

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References for Donepezil hydrochloride

References are publications that support the biological activity of the product.

Hugon Impact of donepezil on brain glucose metabolism assessed using [¹⁸F]2-fluoro-2-deoxy-D-glucose positron emission tomography imaging in a mouse model of Alzheimer's disease induced by intracerebroventricular injection of amyloid-beta peptid Front.Neurosci. 2022 PMID: 35281502
Kim Donepezil regulates LPS and Aβ-stimulated neuroinflammation through MAPK/NLRP3 inflammasome/STAT3 signaling. Int.J.Mol.Sci. 2021 PMID: 34638977
Droguerre Efficacy of THN201, a combination of donepezil and mefloquine, to reverse neurocognitive deficits in Alzheimer's disease. Front.Neurosci. 2020 PMID: 32612499
Romberg Impaired attention in the 3xTgAD mouse model of Alzheimer's disease: rescue by donepezil (Aricept). J.Neurosci. 2011 PMID: 21368062
Sugimoto Donepezil hydrochloride (E2020) and other acetylcholinesterase inhibitors. Curr.Med.Chem. 2000 PMID: 10637367
Kryger Structure of acetylcholinesterase complexed with E2020 (Aricept): implications for the design of new anti-Alzheimer drugs. Structure 1999 PMID: 10368299
Sugimoto Synthesis and structure-activity relationships of acetylcholinesterase inhibitors: 1-benzyl-4-[(5,6-dimethoxy-1-oxoindan-2-yl)methyl]piperidine hydrochloride and related compounds. J.Med.Chem. 1995 PMID: 7490731

Citations for Donepezil hydrochloride (2)

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Citations are publications that use Bio-Techne products. Selected citations for Donepezil hydrochloride include:

Rennekamp et al. (2016) σ1 receptor ligands control a switch between passive and active threat responses. Nat Chem Biol. 12:552
PMID: 27239788
Morris et al. (2015) Network dysfunction in α-synuclein transgenic mice and human Lewy body dementia. Ann Clin Transl Neurol. 2:1012
PMID: 26732627

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