Disulfiram

Product Description

Disulfiram is an inhibitor of aldehyde dehydrogenase that displays antialcoholism activity. Shown to reversibly stimulate Ca²⁺-ATPase activity and inhibit V-ATPase (EC₅₀ = 26 μM). Also inhibits expression of MMP-2 and MMP-9 and displays anti-invasive activity. Pyroptosis inhibitor; binds gasdermin D (GSDMD) and inhibits pore formation. Also inhibits SARS-CoV-2 main protease (M^pro; IC₅₀ = 9.35 μM) and inhibits viral replication in Vero cells in vitro.

Solubility

Preparing Stock Solutions for Disulfiram

The following data is based on the product molecular weight 296.54

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.37 mL	16.86 mL	33.72 mL
5 mM	0.67 mL	3.37 mL	6.74 mL
10 mM	0.34 mL	1.69 mL	3.37 mL
50 mM	0.07 mL	0.34 mL	0.67 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Jin Structure of M^pro from SARS-CoV-2 and discovery of its inhibitors. Nature 2020 PMID: 32272481
• Pandeya Gasdermin D (GSDMD) as a new target for the treatment of infection. Medchemcomm 2019 PMID: 31191857
• Fisher The ethanol metabolite acetaldehyde increases paracellular drug permeability in vitro and oral bioavailability in vivo. J.Pharmacol.Exp.Ther. 2010 PMID: 19820208
• Johnson Identification of inhibitors of vacuolar proton-translocating ATPase pumps in yeast by high-throughput screening flow cytometry. Anal.Biochem. 2009 PMID: 20018164
• Cho Disulfiram suppresses invasive ability of osteosarcoma cells via the inhibition of MMP-2 and MMP-9 expression. J.Biochem.Mol.Biol. 2007 PMID: 18047805
• Starling Stimulation of the Ca²⁺-ATPase of sarcoplasmic reticulum by disul. Biochem.J. 1996 PMID: 8947473