Disulfiram

Name: Disulfiram
Brand: Tocris Bioscience
SKU: 3807
Rating: 5 (1 reviews)

Catalog # 3807 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (3)

COA / SDS

Reversibly stimulates SERCA Ca2+-ATPase; displays a range of other activities

Key Product Details

Description:	Reversibly stimulates SERCA Ca²⁺-ATPase; displays a range of other activities
Alternative Names:	Tetraethylthiuram disulfide
Chemical Name:	Bis(diethylthiocarbamyoyl)disulfide
Molecular Weight:	296.54

View Full Technical Details for Disulfiram

Technical Data
Citations (3) / Reviews (1)

Biological Activity for Disulfiram

Disulfiram is an inhibitor of aldehyde dehydrogenase that displays antialcoholism activity. Shown to reversibly stimulate Ca²⁺-ATPase activity and inhibit V-ATPase (EC₅₀ = 26 μM). Also inhibits expression of MMP-2 and MMP-9 and displays anti-invasive activity. Pyroptosis inhibitor; binds gasdermin D (GSDMD) and inhibits pore formation. Also inhibits SARS-CoV-2 main protease (M^pro; IC₅₀ = 9.35 μM) and inhibits viral replication in Vero cells in vitro.

Technical Data for Disulfiram

M. Wt	296.54
Formula	C₁₀H₂₀N₂S₄
Storage	Store at +4°C
CAS Number	97-77-8
PubChem ID	3117
InChi Key	AUZONCFQVSMFAP-UHFFFAOYSA-N
Smiles	S=C(SSC(N(CC)CC)=S)N(CC)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Disulfiram

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	5.93	20
	ethanol	29.65	100

Preparing Stock Solutions for Disulfiram

The following data is based on the product molecular weight 296.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.37 mL	16.86 mL	33.72 mL
5 mM	0.67 mL	3.37 mL	6.74 mL
10 mM	0.34 mL	1.69 mL	3.37 mL
50 mM	0.07 mL	0.34 mL	0.67 mL

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References for Disulfiram

References are publications that support the biological activity of the product.

Jin Structure of M^pro from SARS-CoV-2 and discovery of its inhibitors. Nature 2020 PMID: 32272481
Pandeya Gasdermin D (GSDMD) as a new target for the treatment of infection. Medchemcomm 2019 PMID: 31191857
Fisher The ethanol metabolite acetaldehyde increases paracellular drug permeability in vitro and oral bioavailability in vivo. J.Pharmacol.Exp.Ther. 2010 PMID: 19820208
Johnson Identification of inhibitors of vacuolar proton-translocating ATPase pumps in yeast by high-throughput screening flow cytometry. Anal.Biochem. 2009 PMID: 20018164
Cho Disulfiram suppresses invasive ability of osteosarcoma cells via the inhibition of MMP-2 and MMP-9 expression. J.Biochem.Mol.Biol. 2007 PMID: 18047805
Starling Stimulation of the Ca²⁺-ATPase of sarcoplasmic reticulum by disul. Biochem.J. 1996 PMID: 8947473

Citations for Disulfiram (3)

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Citations are publications that use Bio-Techne products. Selected citations for Disulfiram include:

Franz S et al. (2020) A phenotypic screening platform utilising human spermatozoa identifies compounds with contraceptive activity. Elife. 9
PMID: 31987071
Chan et al. (2016) HIV-1 latency and virus production from unintegrated genomes following direct infection of resting CD4 T cells. PLoS One. 13:1
PMID: 26728316
Ahadome et al. (2016) Aldehyde dehydrogenase inhibition blocks mucosal fibrosis in human and mouse ocular scarring. JCI Insight. 1:e87001
PMID: 27699226

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Customer Reviews for Disulfiram (1)

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Irreversible inhibitor of Aldh1 with IC50 initial at 50 uM

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: recombinant mouse Aldh1

Recombinant Aldh1 was used in a standard fluorometric reporter assay with NAD+ as a cofactor, reaction velocity measured as relative changes in fluorescence. Disulfiram was added immediately prior to assessing activity. We noted decreasing velocity rates over time supporting this as a time-dependent/ irreversible inhibitor of Aldh enzymes.

Pre-incubation of recombinant enzyme will lead to full depletion of activity. Observed IC50 (initial) at approximately 50 uM.

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