Dihydro-β-erythroidine hydrobromide

Name: Dihydro-β-erythroidine hydrobromide
Brand: Tocris Bioscience
SKU: 2349

Catalog # 2349 | Tocris Bioscience a Bio-Techne Brand

Citations (30)

COA / SDS

α4β2, muscle type and Torpedo nAChR antagonist

Key Product Details

Description:	α₄β₂, muscle type and Torpedo nAChR antagonist
Alternative Names:	DHβE
Chemical Name:	(2S,13bS)-2-Methoxy-2,3,5,6,8,9,10,13-octahydro-1H,12H-benzo[i]pyrano[3,4-g]indolizin-12-one hydrobromide
Purity:	≥98% (HPLC)
Molecular Weight:	356.26

View Full Technical Details for Dihydro-β-erythroidine hydrobromide

Technical Data
Citations (30) / Reviews

Biological Activity for Dihydro-β-erythroidine hydrobromide

Dihydro-β-erythroidine hydrobromide is a member of the Erythrina alkaloids, it is a competitive nicotinic acetylcholine receptor antagonist with moderate selectivity for the neuronal α4 receptor subunit (IC₅₀ values are 0.19 and 0.37 μM for α4β4 and α4β2 receptors, respectively). Antagonizes behavioral effects of nicotine in vivo. Dihydro-β-erythroidine blocks excitation of striatal GABAergic neurons, which completely suppress polysynaptic inhibition between striatal cholinergic interneurons. Dihydro-β-erythroidine hydrobromide has antidepressive-like effects in mice (forced swim and mouse suspension). Orally bioavailable.

Compound Libraries for Dihydro-β-erythroidine hydrobromide

Dihydro-β-erythroidine hydrobromide is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Dihydro-β-erythroidine hydrobromide

M. Wt	356.26
Formula	C₁₆H₂₁NO₃.HBr
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	29734-68-7
PubChem ID	11957537
InChi Key	GFIGWAJEIMHJJB-LINSIKMZSA-N
Smiles	Br.CO[C@H]1CC=C2CCN3CCC4=C(CC(=O)OC4)[C@]23C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Dihydro-β-erythroidine hydrobromide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	35.63	100
	DMSO	8.91	25

Preparing Stock Solutions for Dihydro-β-erythroidine hydrobromide

The following data is based on the product molecular weight 356.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.81 mL	14.03 mL	28.07 mL
5 mM	0.56 mL	2.81 mL	5.61 mL
10 mM	0.28 mL	1.40 mL	2.81 mL
50 mM	0.06 mL	0.28 mL	0.56 mL

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References for Dihydro-β-erythroidine hydrobromide

References are publications that support the biological activity of the product.

Dorst Polysynaptic inhibition between striatal cholinergic interneurons shapes their network activity patterns in a dopamine-dependent manner. Nat.Commun 2020 PMID: 33037215
Andreasen Antidepressant-like effects of nicotinic acetylcholine receptor antagonists, but not agonists, in the mouse forced swim and mouse tail suspension tests. J.Psychopharmacol. 2009 PMID: 18583432
Sullivan Recurrent Inhibitory Network among Striatal Cholinergic Interneurons. J.Neurosci. 2008 PMID: 18753369
Harvey Multiple determinants of dihydro-β-erythroidine sensitivity on rat neuronal nicotinic receptor α subunits. J.Neurochem. 1996 PMID: 8863500
Damaj In vivo pharmacological effects of dihydro-β-erythroidine, a nicotinic antagonist, in mice. Psychopharmacology 1995 PMID: 7724704
Williams and Robinson Binding of the nicotinic cholinergic antagonist, dihydro-β-erythroidine, to rat brain tissue. J.Neurosci. 1984 PMID: 6502210

Citations for Dihydro-β-erythroidine hydrobromide (30)

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Citations are publications that use Bio-Techne products. Selected citations for Dihydro-β-erythroidine hydrobromide include:

Lozovaya et al. (2349) GABAergic inhibition in dual-transmission cholinergic and GABAergic striatal interneurons is abolished in Parkinson disease. Nat Commun. 9(1):1422
PMID: 29651049
Dorst et al. (2020) Polysynaptic inhibition between striatal cholinergic interneurons shapes their network activity patterns in a dopamine-dependent manner. Nat Commun. 11:5113
PMID: 33037215
Huang et al. (2019) Neural mechanisms of contextual modulation in the retinal direction selective circuit. Nat Commun. 10:2431
PMID: 31160566
Kiguchi (2018) Inhibition of peripheral macrophages by nicotinic acetylcholine receptor agonists suppresses spinal microglial activation and neuropathic pain in mice with peripheral nerve injury. J Neuroinflammation. 15(1):96
PMID: 29587798
Brodnik et al. (2018) Local GABA_A receptor mediated suppression of DA release within the nucleus accumbens. ACS Chem Neurosci. 17:1978
PMID: 30253088
Zhang et al. (2018) Pauses in Cholinergic Interneuron Activity Are Driven by Excitatory Input and Delayed Rectification, with DA Modulation. Neuron. 98:918
PMID: 29754751
Kutlu et al. (2018) Differential effects of α4β2 nicotinic receptor antagonists and partial-agonists on contextual fear extinction in male C57BL/6 mice. Psychopharmacology (Berl). 235:1211
PMID: 29383396
Assous (2017) Differential processing of thalamic information via distinct striatal interneuron circuits. Nat Commun. 8:15860
PMID: 28604688
Govind et al. (2017) Selective and regulated trapping of nicotinic receptor weak base ligands and relevance to smoking cessation. Elife. 6
PMID: 28718768
Sternfeld et al. (2017) Speed and segmentation control mechanisms characterized in rhythmically-active circuits created from spinal neurons produced from genetically-tagged embryonic stem cells. Elife. 6
PMID: 28195039
Jareczek et al. (2017) Plasticity in Brainstem Mechanisms of Pain Modulation by Nicotinic Acetylcholine Receptors in the Rat. Eneuro. 4
PMID: 28197544
Miguel Angel Garcia-Bereguiain et al. (2016) Spontaneous Release Regulates Synaptic Scaling in the Embryonic Spinal Network In Vivo The Journal of Neuroscience. 6:7268
PMID: 27383600
Kunisawa et al. (2016) Nicotine evokes kinetic tremor by activating the inferior olive via α7 nicotinic acetylcholine receptors. Behav.Brain Res.. 314:173
PMID: 27506652
Herman et al. (2016) A cholinergic basal forebrain feeding circuit modulates appetite suppression Nature. 538:253
PMID: 27698417
Mamaligas et al. (2016) Nicotinic and opioid receptor regulation of striatal DA D2-receptor mediated transmission Scientific Reports. 6:37834
PMID: 27886263
Dilger et al. (2015) Absence of plateau potentials in dLGN cells leads to a breakdown in retinogeniculate refinement. J Neurosci. 35:3652
PMID: 25716863
Stouffer et al. (2015) Ins enhances striatal DA release by activating cholinergic interneurons and thereby signals reward. Nat Commun. 6:8543
PMID: 26503322
Saunders et al. (2015) Corelease of acetylcholine and GABA from cholinergic forebrain neurons. J Neurosci. 4
PMID: 25723967
Ngolab et al. (2015) Functional Upregulation of α4* Nicotinic Acetylcholine Receptors in VTA GABAergic Neurons Increases Sensitivity to Nicotine Reward. J Neurosci. 35:8570
PMID: 26041923
Yorgason et al. (2015) Greater ethanol inhibition of presynaptic DA release in C57BL/6J than DBA/2J mice: Role of nicotinic acetylcholine receptors. Front Cell Neurosci. 284:854
PMID: 25451295
Elgueta et al. (2015) Acetylcholine induces GABA release onto rod bipolar cells through heteromeric nicotinic receptors expressed in A17 amacrine cells. PLoS One. 9:6
PMID: 25709566
Bader and Diener (2015) Novel aspects of cholinergic regulation of colonic ion transport. Elife. 3:e00139
PMID: 26236483
Morrie and Feller (2015) An Asymmetric Increase in Inhibitory Synapse Number Underlies the Development of a Direction Selective Circuit in the Retina. Neuroscience. 35:9281
PMID: 26109653
Kiss et al. (2014) Nicotinic acetylcholine receptors containing the α7-like subunit mediate contractions of muscles responsible for space positioning of the snail, Helix pomatia L. tentacle. PLoS One. 9:e109538
PMID: 25303328
Zhong et al. (2013) Nicotine elicits prolonged calcium signaling along ventral hippocampal axons. Pharmacol Res Perspect. 8:e82719
PMID: 24349346
Goddard et al. (2012) Gamma oscillations are generated locally in an attention-related midbrain network. Neuron. 73:567
PMID: 22325207
Caiati et al. (2012) Developmental regulation of CB1-mediated spike-time dependent depression at immature mossy fiber-CA3 synapses. Sci Rep. 2:285
PMID: 22368777
Platt et al. (2012) Striatal DA transmission is subtly modified in human A53Tα-synuclein overexpressing mice. PLoS One. 7:e36397
PMID: 22570709
Li et al. (2010) Cytisine induces autonomic cardiovascular responses via activations of different nicotinic receptors. Auton Neurosci. 154:14
PMID: 19887306
Li et al. (2009) Specific subtypes of nicotinic cholinergic receptors involved in sympathetic and parasympathetic cardiovascular responses. Neurosci Lett. 462:20
PMID: 19573576

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