Deazaflavin, Haloalkane

Name: Deazaflavin, Haloalkane
Brand: Tocris Bioscience
SKU: 8987

Tocris Bioscience, part of Bio-Techne | Catalog # 8987

COA / SDS

Photosensitizer for proximity labeling experiments, NHS reactive group

Key Product Details

Description:	Photosensitizer for proximity labeling experiments, NHS reactive group
Chemical Name:	N-(2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)-2-(10-methyl-2,4-dioxo-4,10-dihydropyrimido[4,5-b]quinolin-3(2H)-yl)acetamide
Purity:	≥95% (HPLC)
Molecular Weight:	490.99

View Full Technical Details for Deazaflavin, Haloalkane

Technical Data
Citations

Biological Activity for Deazaflavin, Haloalkane

Deazaflavin, Haloalkane is a deazaflavin-based organic photocatalyst covalently linked to a chloroalkane ligand via a short PEG linker. The conjugate enables site-specific tethering of the photocatalyst to proteins genetically fused to HaloTag^® in live-cell systems.

Upon blue light illumination (~450 nm), the deazaflavin moiety transfers energy to nearby photoactivatable probes (e.g., diazirines, aryl azides), initiating localized covalent labeling of biomolecules in the immediate vicinity of the HaloTag^® fusion protein. The process is dependent on light exposure, the presence of the reactive probe, and spatial proximity to the tethered photocatalyst.

Deazaflavin, Haloalkane is designed for use in photoproximity labeling experiments that aim to study protein interactions or spatial proteomics in live mammalian cells under mild illumination conditions without the use of metal-based photocatalysts.

HaloTag^® is a registered trademark of Promega Corporation.

Technical Data for Deazaflavin, Haloalkane

M. Wt	490.99
Formula	C₂₄H₃₁ClN₄O₅
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	CFRUFYBIYIESMB-UHFFFAOYSA-N
Smiles	O=C1N(C(C2=CC3=CC=CC=C3N(C2=N1)C)=O)CC(NCCOCCOCCCCCCCl)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Deazaflavin, Haloalkane

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.91	10

Preparing Stock Solutions for Deazaflavin, Haloalkane

The following data is based on the product molecular weight 490.99.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	20.37 mL	101.84 mL	203.67 mL
0.5 mM	4.07 mL	20.37 mL	40.73 mL
1 mM	2.04 mL	10.18 mL	20.37 mL
5 mM	0.41 mL	2.04 mL	4.07 mL

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References for Deazaflavin, Haloalkane

References are publications that support the biological activity of the product.

Crocker Energy-transfer photoproximity labelling in live cells using an organic cofactor. Nat.Chem. 2025 PMID: 40962911