DADPS Biotin Azide New
Tocris Bioscience, part of Bio-Techne | Catalog # 7966
Acid cleavable biotin capture reagent, with an azide group as a click handle for conjugation
Key Product Details
Description
Acid cleavable biotin capture reagent, with an azide group as a click handle for conjugation
Product Description
DADPS Biotin Azide is an acid cleavable biotin capture reagent, with an azide group as a click handle for conjugation. The biotin portion strongly binds to avidin or streptavidin to allow isolation of specific, tagged proteins or cells from a complex biological mixture. The DADPS component is a cleavable linker, which can be cleaved under very mild acidic treatment (such as 10% formic acid), unlike traditional biotin probes that require harsh conditions (which can damage samples) to break the streptavidin bond.Product Specifications
Molecular Weight
886.19
Formula
C43H67N7O9SSi
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-[2-[[[(6-Azidohexyl)oxy]diphenylsilyl]oxy]-2-methylpropyl]-21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-17-oxo-4,7,10,13-tetraoxa-16-azaheneicosanamide
CAS Number
1260247-50-4
PubChem ID
132505710
InChI Key
CSHHHVOFXKFHMG-PCXFPAGCSA-N
SMILES
[Si](OC(CNC(CCOCCOCCOCCOCCNC(CCCC[C@H]1[C@@]2([C@](CS1)(NC(=O)N2)[H])[H])=O)=O)(C)C)(OCCCCCCN=[N+]=[N-])(C3=CC=CC=C3)C4=CC=CC=C4
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 88.62 | 100 |
Preparing Stock Solutions
for DADPS Biotin Azide
The following data is based on the product molecular weight 886.19
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.13 mL | 5.64 mL | 11.28 mL |
| 5 mM | 0.23 mL | 1.13 mL | 2.26 mL |
| 10 mM | 0.11 mL | 0.56 mL | 1.13 mL |
| 50 mM | 0.02 mL | 0.11 mL | 0.23 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Li Benchmarking cleavable biotin tags for peptide-centric chemoproteomics. J.Proteome Res. 2022 PMID: 35467356
- Lai Quantitative site-specific chemoproteomic profiling of protein lipoylation. JACS 2022 PMID: 35648456
- Ruan Proteome-wide deconvolution of drug targets and binding sites by lysine reactivity profiling. Anal.Chem. 2022 PMID: 35147412
- McConnell Maleimide-based chemical proteomics for quantitative analysis of cysteine reactivity. J.Am.Soc.Mass Spectrom. 2020 PMID: 32573231
- Rabalski Evaluation of chemically-cleavable linkers for quantitative mapping of small molecule-cysteinome reactivity. ACS Chem.Biol. 2019 PMID: 31430117
- Li Characterization of the artemisinin binding site for translationally controlled tumor protein (TCTP) by bioorthogonal click chemistry. Bioconj.Chem. 2016 PMID: 27998071
Product Specific Notices for DADPS Biotin Azide
For research use only
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