CLP 257

Name: CLP 257
Brand: Tocris Bioscience
SKU: 5242

Tocris Bioscience, part of Bio-Techne | Catalog # 5242

Citations (9)

COA / SDS

Selective KCC2 activator

Key Product Details

Description:	Selective KCC2 activator
Chemical Name:	(5Z)-5-[(4-Fluoro-2-hydroxyphenyl)methylene]-2-(tetrahydro-1-(2H)-pyridazinyl)-4(5H)-thiazolone
Purity:	≥98% (HPLC)
Molecular Weight:	307.34

View Full Technical Details for CLP 257

Technical Data
Citations

Biological Activity for CLP 257

CLP 257 is a selective KCC2 K⁺-Cl^- cotransporter activator (EC₅₀ = 616 nM). Exhibits selectivity for KCC2 over other KCC family members, NKCC1 and GABA_A receptors, as well as 55 other receptors. Enhances Cl^- transport and increases KCC2 plasma membrane expression in spinal slices with reduced KCC2 function. Alleviates hypersensitivity in rats with peripheral nerve injury.

Technical Data for CLP 257

M. Wt	307.34
Formula	C₁₄H₁₄FN₃O₂S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1181081-71-9
PubChem ID	44188931
InChi Key	SKCADXVKQRCWTR-GHXNOFRVSA-N
Smiles	OC1=C(/C=C2/C(N=C(N3CCCCN3)S2)=O)C=CC(F)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CLP 257

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.73	100

Preparing Stock Solutions for CLP 257

The following data is based on the product molecular weight 307.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.25 mL	16.27 mL	32.54 mL
5 mM	0.65 mL	3.25 mL	6.51 mL
10 mM	0.33 mL	1.63 mL	3.25 mL
50 mM	0.07 mL	0.33 mL	0.65 mL

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References for CLP 257

References are publications that support the biological activity of the product.

Gagnon Chloride extrusion enhancers as novel therapeutics for neurological diseases. Nat.Med. 2013 PMID: 24097188