Chloroquine Inhibitor

Chloroquine Inhibitor [NBP2-29386] - TLR9/NF-kB/SEAPorter™ HEK 293 and TLR2/NF-kB/SEAPorter™ HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with different concentrations of chloroquine (0.05, 0.1, 0.5, 1, 5 and 10 uM) for 30 min. Cell were then stimulated with 10ug/ml CpG [A] or 20 ng/ml MALP-2 [B] for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter™ Assay Kit.

Chloroquine Inhibitor [NBP2-29386] - TLR9/NF-kB/SEAPorter™ HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with various concentrations of chloroquine for 30 min, followed by stimulation with 10 ug/ml CpG for 24 h. SEAP was analyzed using SEAPorter™ Assay Kit. Dose-responsive percent inhibition of each sample well was calculated to yield the chloroquine IC50 value.

Chloroquine Inhibitor [NBP2-29386]