CDK8/19i

Name: CDK8/19i
Brand: Tocris Bioscience
SKU: 7372

Tocris Bioscience, part of Bio-Techne | Catalog # 7372

COA / SDS

Potent and selective CDK8 and CDK19 inhibitor; maintains pluripotency of mouse PSCs in culture

Key Product Details

Description:	Potent and selective CDK8 and CDK19 inhibitor; maintains pluripotency of mouse PSCs in culture
Chemical Name:	N-Propyl-3-(4-pyridinyl)imidazo[1,2-b]pyridazin-6-amine
Purity:	≥98% (HPLC)
Molecular Weight:	253.3

View Full Technical Details for CDK8/19i

Technical Data
Citations

Biological Activity for CDK8/19i

CDK8/19i is a potent and selective CDK8 and CDK19 inhibitor (IC₅₀ values are 2.9 and 14.1 nM, respectively). CDK8/19i maintains pluripotency of mouse PSCs in culture. It induces the formation of dome-shaped colonies and upregulates Nanog-GFP reporter and endogenous Nanog expression in mouse ESCs in vitro.

Compound Libraries for CDK8/19i

CDK8/19i is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for CDK8/19i

M. Wt	253.3
Formula	C₁₄H₁₅N₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	923596-52-5
PubChem ID	68488430
InChi Key	XTMROGQLIVGYKF-UHFFFAOYSA-N
Smiles	CCCNC1=NN2C(C=C1)=NC=C2C3=CC=NC=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CDK8/19i

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	25.33	100
	ethanol	25.33	100

Preparing Stock Solutions for CDK8/19i

The following data is based on the product molecular weight 253.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.95 mL	19.74 mL	39.48 mL
5 mM	0.79 mL	3.95 mL	7.90 mL
10 mM	0.39 mL	1.97 mL	3.95 mL
50 mM	0.08 mL	0.39 mL	0.79 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for CDK8/19i

References are publications that support the biological activity of the product.

Lynch Global hyperactivation of enhancers stabilizes human and mouse naive pluripotency through inhibition of CDK8/19 Mediator kinases. Nat.Cell.Bio. 2020 PMID: 32989249