Are R&D Systems Caspase Inhibitors irreversible?

Yes, the majority of R&D Systems Caspase Inhibitors have a Fluoromethyl ketone (FMK) functional group on the C-terminus of the peptide, and act as effective irreversible inhibitors with no added cytotoxic effects. Inhibitors synthesized with a benzyloxycarbonyl group (also known as BOC or Z) at the N-terminus and O-methyl side chains exhibit enhanced cellular permeability. R&D Systems also offers a General Caspase Inhibitor, Q-VD-OPh, Catalog # OPH001, as well as a FITC-conjugated pan-caspase inhibitor (ApoStat), Catalog # FMK012, which are both cell-permeable, irreversible inhibitors of caspase activity.

Does R&D Systems offer a negative control for Caspase Inihibitors with benzyloxycarbonyl group (Z-) at the N-terminus and the FMK functional group at the C-terminus?

Yes, R&D Systems offers Caspase Inhibitor Control Z-FA-FMK, Catalog # FMKC01, which is an inhibitor of cathepsins B and L but not caspases, and has been used in several systems as a negative control for peptide inhibitors of caspases.

Caspase-4 Inhibitor Z-YVAD-FMK

Name: Caspase-4 Inhibitor Z-YVAD-FMK
Brand: R&D Systems
SKU: FMK005

R&D Systems, part of Bio-Techne | Catalog # FMK005

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Product Datasheet / COA / SDS

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Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Cell permeable fluoromethyl ketone (FMK)-derivatized peptides act as effective irreversible Caspase inhibitors with no cytotoxic effects and, therefore, are useful tools for studying Caspase activity.

Conjugate

Unconjugated

View all Caspase-4 Small Molecules and Peptides »

Formulation, Preparation and Storage

FMK005

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Caspase-4

Caspase-11 (also known as as Caspase-4) is a cysteine-aspartic acid protease that functions in apoptosis initiation and the activation of proinflammatory cytokines. Pro-Caspase-11 is expressed as a zymogen which is cleaved into two subunits, p20 and p10. It is activated by an autocatalytic mechanism or by Caspase-8 mediated cleavage in response to ER stress or bacterial infection. Its expression and activation can be induced by exposure to LPS. Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta.

Alternate Names

CASP4, Caspase4, ICE(rel)II, ICH-2, Mih1/TX, TX

Entrez Gene IDs

837 (Human)

Gene Symbol

CASP4

Additional Caspase-4 Products

Product Documents for Caspase-4 Inhibitor Z-YVAD-FMK

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Product Specific Notices for Caspase-4 Inhibitor Z-YVAD-FMK

For research use only

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