C28

Name: C28
Brand: Tocris Bioscience
SKU: 7546

Tocris Bioscience, part of Bio-Techne | Catalog # 7546

COA / SDS

Potent LMTK3 inhibitor

Key Product Details

Description:	Potent LMTK3 inhibitor
Chemical Name:	6-(Pyridin-4-yl)-3-(3-(trifluoromethoxy)phenyl)imidazo[1,2-b]pyridazine
Purity:	≥98% (HPLC)
Molecular Weight:	356.31

View Full Technical Details for C28

Technical Data
Citations

Biological Activity for C28

C28 is a potent lemur tyrosine kinase 3 (LMTK3) inhibitor (IC₅₀ = 67 nM). Disrupts interaction of LMTK3 and HSP90 leading to degradation of LMTK3. Also inhibits CLK, DYRK1α, IRAK4 and HIPK2 (IC₅₀ values are 5 nM, 6 nM, 41 nM and 48 nM respectively). C28 inhibits growth and increases apoptosis in breast cancer cell lines (IC₅₀ = 6.5-14.77 μM) and reduces growth of tumors in xenograft and transgenic mouse models of breast cancer. Also reduces levels of ERα.

Licensing Information

Sold with kind permission of the Giamas lab, University of Sussex

Technical Data for C28

M. Wt	356.31
Formula	C₁₈H₁₁F₃N₄O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2764850-23-7
PubChem ID	138454301
InChi Key	SFBZOJORTROAIE-UHFFFAOYSA-N
Smiles	FC(F)(OC1=CC(C2=CN=C3C=CC(C4=CC=NC=C4)=NN32)=CC=C1)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for C28

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	17.82	50
	ethanol	7.13	20

Preparing Stock Solutions for C28

The following data is based on the product molecular weight 356.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.61 mL	28.07 mL	56.13 mL
2.5 mM	1.12 mL	5.61 mL	11.23 mL
5 mM	0.56 mL	2.81 mL	5.61 mL
25 mM	0.11 mL	0.56 mL	1.12 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for C28

References are publications that support the biological activity of the product.

Ditsiou The structure-function relationship of oncogenic LMTK3. Sci.Adv. 2020 PMID: 33188023

C28