Brefeldin A

Name: Brefeldin A
Brand: Tocris Bioscience
SKU: 1231
Rating: 5 (1 reviews)

Catalog # 1231 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (10)

COA / SDS

Disrupts protein translocation to Golgi

Key Product Details

Description:	Disrupts protein translocation to Golgi
Chemical Name:	1,6,7,8,9,11aβ,12,13,14,14aα-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent[f]oxacyclotridecin-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	280.36

View Full Technical Details for Brefeldin A

Technical Data
Citations (10) / Reviews (1)

Biological Activity for Brefeldin A

Brefeldin A is a reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor (ARF1) to the Golgi apparatus and inhibits GDP-GTP exchange. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs). Antifungal.

Technical Data for Brefeldin A

M. Wt	280.36
Formula	C₁₆H₂₄O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	20350-15-6
PubChem ID	6436187
InChi Key	KQNZDYYTLMIZCT-SZXCEISTSA-N
Smiles	O[C@@H]([C@H]1[C@H](/C=C/CCC[C@H](C)O2)C[C@H](O)C1)/C=C/C2=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Brefeldin A

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	14.02	50

Preparing Stock Solutions for Brefeldin A

The following data is based on the product molecular weight 280.36.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	7.13 mL	35.67 mL	71.34 mL
2.5 mM	1.43 mL	7.13 mL	14.27 mL
5 mM	0.71 mL	3.57 mL	7.13 mL
25 mM	0.14 mL	0.71 mL	1.43 mL

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References for Brefeldin A

References are publications that support the biological activity of the product.

Yu Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 2015 PMID: 25658371
Ding Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 2007 PMID: 17135238
Morinaga Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 1996 PMID: 8917509
Tsai Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 1994 PMID: 8159707
Ktistakis Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 1992 PMID: 1549178

Citations for Brefeldin A (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Brefeldin A include:

Shemorry et al. (2019) Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress. Elife. 8
PMID: 31453810
Hossain et al. (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget. 9:11707
PMID: 29545931
Fauster et al. (2018) Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Cell Death Differ. 2018;
PMID: 30237509
Nieuwenhuijze et al. (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol. 9:2234
PMID: 30323813
Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep. 21(10):2895
PMID: 29212034
Bowen et al. (2017) Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines. Elife. 6
PMID: 28875935
Halff et al. (2014) A novel mechanism for nicotinic potentiation of glutamatergic synapses. J Neurosci. 34:2051
PMID: 24501347
Saba et al. (2014) Rhinovirus-induced macrophage cytokine expression does not require endocytosis or replication. Am J Respir Cell Mol Biol. 50:974
PMID: 24783958
Grassin-Delyle et al. (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res. 14:134
PMID: 24266887
Jing et al. (2011) Concerted actions of NHERF2 and WNK4 in regulating TRPV5. J Neurosci. 404:979
PMID: 21187068

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Customer Reviews for Brefeldin A (1)

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Usage Information

Name: Anonymous

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: KMS11 or OPM2

0.2 μg/ml

We treated cells for 16 hr.

PMID: 31453810 Reference

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