BRD 0539
Tocris Bioscience, part of Bio-Techne | Catalog # 7153
Cell permeable and reversible Cas9 inhibitor
Key Product Details
Description
Cell permeable and reversible Cas9 inhibitor
Product Description
BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC50 = 22 μM) and in an eGFP disruption assay (apparent EC50 = 11 μM). Allows dose and temporal control of SpCas9-based systems.Licensing Information
Sold under licence from the Broad InstituteProduct Specifications
Molecular Weight
452.54
Formula
C25H25FN2O3S
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(3aR,4R,9bR)-8-(2-Fluorophenyl)-2,3,3a,4,5,9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3,2-c]quinoline-4-methanol
CAS Number
1403838-79-8
PubChem ID
54666326
InChI Key
CZOIXFMISSBIJL-DCEDVJGZSA-N
SMILES
[H][C@]12CCN([C@@]1([H])C1=CC(=CC=C1N[C@H]2CO)C1=C(F)C=CC=C1)[S](=O)(=O)C1=CC=C(C)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 45.25 | 100 | |
| Ethanol | 45.25 | 100 |
Preparing Stock Solutions
for BRD 0539
The following data is based on the product molecular weight 452.54
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.21 mL | 11.05 mL | 22.10 mL |
| 5 mM | 0.44 mL | 2.21 mL | 4.42 mL |
| 10 mM | 0.22 mL | 1.10 mL | 2.21 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Maji A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9. Cell 2019 PMID: 31051099
Product Specific Notices for BRD 0539
For research use only
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