Biotin-PEG4-Diazirine New

Name: Biotin-PEG4-Diazirine
Brand: Tocris Bioscience
SKU: 8994

Tocris Bioscience, part of Bio-Techne | Catalog # 8994

COA / SDS

Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle

Key Product Details

Description:	Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle
Chemical Name:	1-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)-N-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)-3,6,9,12-tetraoxapentadecan-15-amide
Purity:	≥95% (HPLC)
Molecular Weight:	688.75

View Full Technical Details for Biotin-PEG4-Diazirine

Technical Data
Citations

Biological Activity for Biotin-PEG4-Diazirine

Key information:Biotin-PEG4-Diazirine is a cell-permeable photo-crosslinking probe for elucidating protein interactome of polyarginine cell-penetrating peptides and mapping cell surfaceome through deazaflavin-diazirine energy-transfer labelling (DarT labelling). Upon UV irradiation and in the presence of deazaflavin as an organic photocatalyst, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby proteins. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. Suitable for live-cell applications.

Used for: photocatalytic proximity labelling.

Application: deazaflavin-diazirine energy-transfer labelling (DarT labelling) followed by affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures.

Technical Data for Biotin-PEG4-Diazirine

M. Wt	688.75
Formula	C₃₀H₄₃F₃N₆O₇S
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	MGUZAQIDWYWQHB-DPZBCOQUSA-N
Smiles	O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCOCCOCCOCCOCCC(NCC3=CC=C(C4(N=N4)C(F)(F)F)C=C3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Biotin-PEG4-Diazirine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	34.44	50 with gentle warming

Preparing Stock Solutions for Biotin-PEG4-Diazirine

The following data is based on the product molecular weight 688.75.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	2.90 mL	14.52 mL	29.04 mL
2.5 mM	0.58 mL	2.90 mL	5.81 mL
5 mM	0.29 mL	1.45 mL	2.90 mL
25 mM	0.06 mL	0.29 mL	0.58 mL

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References for Biotin-PEG4-Diazirine

References are publications that support the biological activity of the product.

Crocker Energy-transfer photoproximity labelling in live cells using an organic cofactor. Nature Chemistry 2025 PMID: 40962911