Biotin BromoCatch™ Ligand

Name: Biotin BromoCatch™ Ligand
Brand: Tocris Bioscience
SKU: 8939

Tocris Bioscience, part of Bio-Techne | Catalog # 8939

COA / SDS

A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.

Key Product Details

Description:	A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
Chemical Name:	N-((17R)-17-((6S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-16-oxo-3,6,9,12-tetraoxa-15-azanonadecyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Purity:	≥95% (HPLC)
Molecular Weight:	908.15

View Full Technical Details for Biotin BromoCatch™ Ligand

Technical Data
Citations

Biological Activity for Biotin BromoCatch™ Ligand

Biotin BromoCatch™ Ligand is a covalent probe designed for the selective labeling and enrichment of proteins tagged with the BromoCatch™ self-labeling tag. Based on a para-acrylamide-functionalized bump-JQ1 scaffold, the BromoCatch™ ligand enables irreversible and highly specific covalent attachment to the engineered cysteine residue in the BromoCatch-tagged protein. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. This probe demonstrates exceptional target selectivity, confirmed via intact protein mass spectrometry and western blotting, and shows no background labeling in untransfected or wild-type samples even at micromolar concentrations. Biotin BromoCatch™ Ligand expands the utility of the BromoCatch™ platform for biochemical workflows including proteomics, immunoprecipitation, and interaction studies.

Please refer to this protocol for further information on how to use this product.

This product is part of an early adopter program.

BromoCatch™ is a trademark of Bio-Techne Corp.

Licensing Information

Sold under exclusive licence from the University of Dundee

Technical Data for Biotin BromoCatch™ Ligand

M. Wt	908.15
Formula	C₄₄H₆₁N₉O₈S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	BZQKBFKRTBEEFV-HQANLXDBSA-N
Smiles	CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCOCCNC(CCCC[C@H]4[C@](NC(N5)=O)([H])[C@]5([H])CS4)=O)=O)CC)C6=NN=C(C)N26

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Biotin BromoCatch™ Ligand

References are publications that support the biological activity of the product.

Rodriguez-Rios BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv