Auxinole

Name: Auxinole
Brand: Tocris Bioscience
SKU: 7894

Tocris Bioscience, part of Bio-Techne | Catalog # 7894

COA / SDS

Auxin antagonist of TIR1/AFB receptors; also OsTIR1 inhibitor in auxin-inducible degron (AID) system

Key Product Details

Description:	Auxin antagonist of TIR1/AFB receptors; also OsTIR1 inhibitor in auxin-inducible degron (AID) system
Chemical Name:	α-[2-(2,4-Dimethylphenyl)-2-oxoethyl]-1H-indole-3-acetic acid
Purity:	≥98% (HPLC)
Molecular Weight:	321.37

View Full Technical Details for Auxinole

Technical Data
Citations

Biological Activity for Auxinole

Auxinole is an auxin antagonist of TIR1/AFB receptors. Binds to TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and inhibit auxin-responsive gene expression. Auxinole competitively inhibits various auxin responses in planta. Auxinole also inhibits OsTIR1 that suppresses leaky degradation of degron-fused proteins. Can be used as a control compound with 5-Ph-IAA (Cat. No. 7392) in the AID2 degron system to switch off degradation.

Technical Data for Auxinole

M. Wt	321.37
Formula	C₂₀H₁₉NO₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	86445-22-9
PubChem ID	13077496
InChi Key	HGUYAIJBXSQXGV-UHFFFAOYSA-N
Smiles	O=C(O)C(C1=CNC=2C=CC=CC21)CC(=O)C3=CC=C(C=C3C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Auxinole

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	32.14	100
	ethanol	6.43	20 with gentle warming

Preparing Stock Solutions for Auxinole

The following data is based on the product molecular weight 321.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.11 mL	15.56 mL	31.12 mL
5 mM	0.62 mL	3.11 mL	6.22 mL
10 mM	0.31 mL	1.56 mL	3.11 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

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References for Auxinole

References are publications that support the biological activity of the product.

Yesbolatova Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods 2019 PMID: 31026591
Hayashi Rational design of an auxin antagonist of the SCF(TIR1) auxin receptor complex. ACS Chem.Biol. 2012 PMID: 22234040