Asunaprevir

Product Description

Asunaprevir is a potent HCV NS3 protease inhibitor (IC₅₀ = 0.2-3.5 nM). Asunaprevir can be used to control CRIPSR-Cas9 gene editing via the small molecule-assisted shut-off (SMASh) technique, which involves fusion of Cas9 to a peptide comprising a protease domain and a degron tag. Asuprenavir blocks the protease-mediated removal of the degron tag leading to degradation of Cas9 through proteasome and autophagolysosome pathways. It can also be used as a resversible switch to control CAR (chimeric antigen receptor)-T cells, where the CAR has been modified by insertion of a hepatitis C virus nonstructural protein 3 protease (HCV-NS3) between the scFv and the hinge domains. Also inhibits SARS-CoV-2 M^pro activity (IC₅₀ = 15-48.4 μM).

Solubility

Preparing Stock Solutions for Asunaprevir

The following data is based on the product molecular weight 748.29

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.34 mL	6.68 mL	13.36 mL
5 mM	0.27 mL	1.34 mL	2.67 mL
10 mM	0.13 mL	0.67 mL	1.34 mL
50 mM	0.03 mL	0.13 mL	0.27 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Cao A reversible chemogenetic switch for chimeric antigen receptor T cells. Angew.Chem.Int.Ed.Engl. 2022 PMID: 34783141
• Wu A small molecule-controlled Cas9 repressible system. Mol.Ther.Nucleic Acids 2020 PMID: 32000033
• Bafna Hepatitis C virus drugs that inhibit SARS-CoV-2 papain-like protease synergize with remdesivir to suppress viral replication in cell culture. Cell Rep. 2021 PMID: 33984267
• Chung Tunable and reversible drug control of protein production via a self-excising degron. Nat.Chem.Biol. 2015 PMID: 26214256