AP 20187
Tocris Bioscience, part of Bio-Techne | Catalog # 6297
Chemical inducer of protein dimerization; active in vivo
Key Product Details
Description
Chemical inducer of protein dimerization; active in vivo
Product Description
AP 20187 is a chemical inducer of dimerization (CID) for use in FKBP fusion protein systems. Induces apoptosis in cells expressing fusion proteins consisting of FKBPF36V and the intracellular domain of the Fas receptor, Fas-associated death domain (FADD) or a caspase. Selective for FKBPF36V-Fas fusion proteins over wild-type FKBP. Induces apoptosis of senescent cells expressing caspase-8-FKBP fusion proteins under the control of an Ink4a promoter. Active in vivo.For more information about how AP 20187 may be used, see our protocol.
Product Specifications
Molecular Weight
1482.77
Formula
C82H107N5O20
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2,2'-[[2-[(Dimethylamino)methyl]-1,3-propanediyl]bis[imino(2-oxo-2,1-ethanediyl)oxy-3,1-phenylene[(1R)-3-(3,4-dimethoxyphenyl)propylidene]]] bis[1-[(2S)-1-oxo-2-(3,4,5-trimethoxyphenyl)butyl]-2-piperidinecarboxylate]
CAS Number
195514-80-8
PubChem ID
78357784
InChI Key
NSBGUMKAXUXKGI-BPNHAYRBSA-N
SMILES
O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCC(CN(C)C)CNC(COC4=CC=CC([C@H](OC([C@@H]5CCCCN5C([C@H](C6=CC(OC)=C(OC)C(OC)=C6)CC)=O)=O)CCC7=CC(OC)=C(OC)C=C7)=C4)=O)=O)=CC=C3)CCC8=CC(OC)=C(OC)C=C8
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 148.28 | 100 | |
| Ethanol | 148.28 | 100 |
Preparing Stock Solutions
for AP 20187
The following data is based on the product molecular weight 1482.77
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.67 mL | 3.37 mL | 6.74 mL |
| 5 mM | 0.13 mL | 0.67 mL | 1.35 mL |
| 10 mM | 0.07 mL | 0.34 mL | 0.67 mL |
| 50 mM | 0.01 mL | 0.07 mL | 0.13 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Jacobs StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins. Nat.Methods 2018 PMID: 29967496
- Baker Naturally occurring p16Ink4a-positive cells shorten healthy lifespan. Nature 2016 PMID: 26840489
- Ono Exposure to sequestered self-antigens in vivo is not sufficient for the induction of autoimmune diabetes. PLoS One 2017 PMID: 28257518
- Shariat Adenovirus-mediated transfer of inducible caspases: a novel "death switch" gene therapeutic approach to prostate cancer. Cancer Res. 2001 PMID: 11289132
Product Specific Notices for AP 20187
For research use only
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