ANA 12

Name: ANA 12
Brand: Tocris Bioscience
SKU: 4781

Tocris Bioscience, part of Bio-Techne | Catalog # 4781

Citations (65)

COA / SDS

TrkB receptor antagonist

Key Product Details

Description:	TrkB receptor antagonist
Chemical Name:	N-[2-[[(Hexahydro-2-oxo-1H-azepin-3-yl)amino]carbonyl]phenyl]benzo[b]thiophene-2-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	407.49

View Full Technical Details for ANA 12

Technical Data
Citations

Biological Activity for ANA 12

ANA 12 is a TrkB receptor antagonist. Demonstrates a 2-site mode of action, preventing activation by BDNF non-competitively (IC₅₀ values are 45.6 nM and 41.1 μM for the high and low affinity sites respectively). Displays no effect on TrkA and TrkC in an assay of neurite outgrowth. Demonstrates antidepressant properties. Also exhibits anti-seizure effects in an ischemia-induced neonatal seizure model by blocking down-regulation of KCC2.

Compound Libraries for ANA 12

ANA 12 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ANA 12

M. Wt	407.49
Formula	C₂₂H₂₁N₃O₃S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	219766-25-3
PubChem ID	2799722
InChi Key	TUSCYCAIGRVBMD-UHFFFAOYSA-N
Smiles	O=C(NC4C(NCCCC4)=O)C1=C(NC(C2=CC3=C(C=CC=C3)S2)=O)C=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ANA 12

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	8.15	20

Preparing Stock Solutions for ANA 12

The following data is based on the product molecular weight 407.49.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	12.27 mL	61.35 mL	122.70 mL
1 mM	2.45 mL	12.27 mL	24.54 mL
2 mM	1.23 mL	6.14 mL	12.27 mL
10 mM	0.25 mL	1.23 mL	2.45 mL

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References for ANA 12

References are publications that support the biological activity of the product.

Carter Dose-dependent reversal of KCC2 hypofunction and phenobarbital-resistant neonatal seizures by ANA12. Sci.Rep. 2018 PMID: 30097625
Cazorla Identification of a low-molecular weight TrkB antagonist with anxiolytic and antidepressant activity in mice. J.Clin.Invest. 2011 PMID: 21505263