Amphotericin B
Tocris Bioscience, part of Bio-Techne | Catalog # 6930
Forms monovalent ion channels in lipid bilyers; antifungal
Key Product Details
Description
Forms monovalent ion channels in lipid bilyers; antifungal
Product Description
Amphotericin B forms nonselective monovalent ion channels within lipid bilayers. Amphotericin B binds ergosterol in the cell membrane. Antifungal and antiprotozoal. Amphotericin B can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells (see below protocol).For more information about how Amphotericin B may be used, see our protocol: 3D Culture of Lung Alveolar Cells
Product Specifications
Molecular Weight
924.09
Formula
C47H73NO17
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
(1R,3S,5R,6R,9R,11R,15S,16R,17R,18S,19E,21E,23E,25E,27E,29E,31E,33R,35S,36R,37S)-33-[(3-Amino-3,6-dideoxy-β-D-mannopyranosyl)oxy]-1,3,5,6,9,11,17,37-octahydroxy-15,16,18-trimethyl-13-oxo-14,39-dioxabicyclo[33.3.1]nonatriaconta-19,21,23,25,27,29,31-heptaene-36-carboxylic acid
CAS Number
1397-89-3
PubChem ID
5280965
InChI Key
APKFDSVGJQXUKY-INPOYWNPSA-N
SMILES
[H][C@]12C[C@@H](O[C@]3([H])O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(O)=O)O2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 18.48 | 20 |
Preparing Stock Solutions
for Amphotericin B
The following data is based on the product molecular weight 924.09
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 5.41 mL | 27.05 mL | 54.11 mL |
| 1 mM | 1.08 mL | 5.41 mL | 10.82 mL |
| 2 mM | 0.54 mL | 2.71 mL | 5.41 mL |
| 10 mM | 0.11 mL | 0.54 mL | 1.08 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Gallis Amphotericin B: 30 years of clinical experience. Rev.Infect.Dis. 1990 PMID: 2184499
- Lewis Current concepts in antifungal pharmacology. Mayo Clin.Proc. 2011 PMID: 21803962
- Muruglia Small-molecule ion channels increase host defences in cystic fibrosis airway epithelia. Nature. 2019 PMID: 30867598
Product Specific Notices for Amphotericin B
For research use only
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