A 769662

Name: A 769662
Brand: Tocris Bioscience
SKU: 3336

Tocris Bioscience, part of Bio-Techne | Catalog # 3336

Citations (119)

COA / SDS

Potent AMPK activator

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Product Description
Product Specifications
Solubility
Preparing Stock Solutions
Calculators
Background References
Product Documents
Specific Notices

Key Product Details

Description

Potent AMPK activator

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Product Description

A 769662 is a potent, reversible AMP-activated protein kinase (AMPK) activator (EC₅₀ = 0.8 μM) that displays selectivity towards β1 subunit-containing heterotrimers. Inhibits fatty acid synthesis (IC₅₀ = 3.2 μM) and decreases plasma glucose and triglyceride levels in vivo. Also inhibits proliferation of mesenchymal stem cells, and impedes reprogramming of mouse embryonic fibroblasts to iPSCs. Activates autophagy.

Product Specifications

Molecular Weight

360.39

Formula

C₂₀H₁₂N₂O₃S

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

6,7-Dihydro-4-hydroxy-3-(2'-hydroxy[1,1'-biphenyl]-4-yl)-6-oxo-thieno[2,3-b]pyridine-5-carbonitrile

CAS Number

844499-71-4

PubChem ID

54708532

InChI Key

CTESJDQKVOEUOY-UHFFFAOYSA-N

SMILES

OC(C1=C(N3)SC=C1C2=CC=C(C4=CC=CC=C4O)C=C2)=C(C#N)C3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	36.04	100

Preparing Stock Solutions for A 769662

The following data is based on the product molecular weight 360.39

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.77 mL	13.87 mL	27.75 mL
5 mM	0.55 mL	2.77 mL	5.55 mL
10 mM	0.28 mL	1.39 mL	2.77 mL
50 mM	0.06 mL	0.28 mL	0.55 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Duca MetF. activates a duodenal Ampk-dependent pathway to lower hepatic glucose production in rats. Nat.Med. 2015 PMID: 25849133
Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID: 28529316
Vazquez-Martin Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells. Cell Cycle 2012 PMID: 2233578
de Meester Role of AMP-activated protein kinase in regulating hypoxic survival and proliferation of mesenchymal stem cells. Cardiovasc.Res. 2014 PMID: 24104879
Cool Identification and characterization of a small molecule AMPK activator that treats key components of type 2 diabetes and the metabolic syndrome. Cell Met.
Scott Thienopyridone drugs are selective activators of AMP-activated protein kinase β1-containing complexes. Chem.Biol. 2008 PMID: 19022182
Sanders Defining the mechanism of activation of AMP-activated protein kinase by the small molecule A-769662, a member of the thienopyridone family. J.Biol.Chem. 2007 PMID: 17728241