8-Bromo-cAMP, sodium salt
Tocris Bioscience, part of Bio-Techne | Catalog # 1140
Cell-permeable cAMP analog
Product Description
8-Bromo-cAMP, sodium salt is a cell-permeable cyclic AMP (cAMP) analog. It activates PKA and has greater resistance to hydrolysis by phosphodiesterase (PDE) than cAMP. It increases differentiation and induces apoptosis in an esophageal cancer cell line. 8-Bromo-cAMP enhances the induction of pluripotency in human fibroblast cells in combination with VPA (valproic acid) (Cat. No. 2815). In combination with IBMX (Cat. No. 2845), it promotes the differentiation of human iPS cell-derived intestinal epithelial cells. It induces cell-based VEGF production for angiogenesis and osteoblastic differentiation in vitro.Product Specifications
Molecular Weight
430.09
Formula
C10H10BrN5NaO6P
Storage
Store at -20°C
Purity
≥99% (HPLC)
Chemical Name
8-Bromoadenosine-3',5'-cyclic monophosphate sodium salt
CAS Number
76939-46-3
PubChem ID
23702958
InChI Key
DMRMZQATXPQOTP-GWTDSMLYSA-M
SMILES
[Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](O)[C@@H](O2)N1C(Br)=NC2=C1N=CN=C2N
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 43.01 | 100 | |
| DMSO | 43.01 | 100 |
Preparing Stock Solutions
for 8-Bromo-cAMP, sodium salt
The following data is based on the product molecular weight 430.09
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.33 mL | 11.63 mL | 23.25 mL |
| 5 mM | 0.47 mL | 2.33 mL | 4.65 mL |
| 10 mM | 0.23 mL | 1.16 mL | 2.33 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.47 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Lo One-day treatment of small molecule 8-bromo-cyclic AMP analogue induces cell-based VEGF production for in vitro angiogenesis and osteoblastic differentiation. J.Tissue.Eng.Regen. Med. 2016 PMID: 24493289
- Wang Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J.Gastroenterol. 2005 PMID: 16425431
- Wang A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev. 2011 PMID: 21120637
- Hei Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs. Mol.Pharmacol. 1991 PMID: 1847496
- Chow Functional modulation of P2X2 receptors by cyclic AMP-dependent protein kinase. J.Neurochem. 1998 PMID: 9603227
- Carranza Protein kinase A induces recruitment of active Na+,K+-ATPase units to the plasma membrane of rat proximal convoluted tubule cells. J.Physiol. 1998 PMID: 9679177
Product Specific Notices for 8-Bromo-cAMP, sodium salt
For research use only
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