5-BrdU

Tocris Bioscience, part of Bio-Techne | Catalog # 5015

Synthetic thymidine analog; used for cell proliferation assays
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Key Product Details

Description

Synthetic thymidine analog; used for cell proliferation assays

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Product Description

5-BrdU is a synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.

Product Specifications

Molecular Weight

307.10

Formula

C9H11BrN2O5

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

5-Bromo-2-deoxyuridine

CAS Number

59-14-3

PubChem ID

6035

InChI Key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

SMILES

O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 15.36 50
DMSO 30.71 100

Preparing Stock Solutions for 5-BrdU

The following data is based on the product molecular weight 307.10

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.26 mL 16.28 mL 32.56 mL
5 mM 0.65 mL 3.26 mL 6.51 mL
10 mM 0.33 mL 1.63 mL 3.26 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

Calculators

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Reconstitution Calculator

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Background References

References are publications that support the biological activity of the product.

Product Documents for 5-BrdU

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 5-BrdU

For research use only

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