5-BDBD

Tocris Bioscience a Bio-Techne Brand

Catalog # 3579

Citations (18)

(1)

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Key Product Details

Description:	Potent and selective P2X₄ antagonist
Chemical Name:	5-(3-Bromophenyl)-1,3-dihydro-2H-benzofuro[3,2-e]-1,4-diazepin-2-one
Purity:	≥99% (HPLC)
Molecular Weight:	355.19

View Full Technical Details for 5-BDBD

Biological Activity for 5-BDBD

5-BDBD is a potent and selective P2X₄ receptor antagonist. Blocks P2X₄-mediated currents in Chinese hamster ovary cells (IC₅₀ = 0.50 μM). Exhibits no significant antagonist effects at other P2X receptors. Reduces long-term potentiation (LTP) in rat hippocampal slices.

Compound Libraries for 5-BDBD

5-BDBD is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for 5-BDBD

M. Wt	355.19
Formula	C₁₇H₁₁BrN₂O₂
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	768404-03-1
PubChem ID	9841560
InChi Key	NKYMVQPXXTZHSF-UHFFFAOYSA-N
Smiles	BrC1=CC(C(C3=C4C2=C(O3)C=CC=C2)=NCC(N4)=O)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5-BDBD

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	35.52	100

Preparing Stock Solutions for 5-BDBD

The following data is based on the product molecular weight 355.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.82 mL	14.08 mL	28.15 mL
5 mM	0.56 mL	2.82 mL	5.63 mL
10 mM	0.28 mL	1.41 mL	2.82 mL
50 mM	0.06 mL	0.28 mL	0.56 mL

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References for 5-BDBD

References are publications that support the biological activity of the product.

Coddou Characterization of the antagonist actions of 5-BDBD at the rat P2X4 receptor. Neurosci Lett. 2018 PMID: 30366010
Casati Cell-autonomous regulation of hematopoietic stem cell cycling activity by ATP. Cell Death Differ. 2011 PMID: 20798687
Donnelly-Roberts Painful purinergic receptors. J.Pharmacol.Exp.Ther. 2008 PMID: 18042830

Citations for 5-BDBD (18)

Showing 1 - 10 of 18 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for 5-BDBD include:

Miguel Cruz & Beall (2020) Extracellular ATP Increases Glucose Metabolism in Skeletal Muscle Cells in a P₂ Receptor Dependent Manner but Does Not Contribute to Palmitate-Induced Insulin Resistance. Front Physiol. 11
PMID: 33101053
Dosch et al. (2019) Connexin-43-dependent ATP release mediates macrophage activation during sepsis. Elife. 8
PMID: 30735126
Dhuna et al. (2019) Ginsenosides Act As Positive Modulators of P2X4 Receptors. Mol Pharmacol. 95:210
PMID: 30545933
Zumerle et al. (2019) Intercellular Calcium Signaling Induced by ATP Potentiates Macrophage Phagocytosis. Cell Rep. 27:1
PMID: 30943393
Uderhardt et al. (2019) Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell. 177:541
PMID: 30955887
Chen et al. (2016) Effect of P2X4R on airway inflammation and airway remodeling in allergic airway challenge in mice. Mol Med Rep. 13:697
PMID: 26648454
Ledderose et al. (2016) Mitochondrial Dysfunction, Depleted Purinergic Signaling, and Defective T Cell Vigilance and Immune Defense. J Infect Dis. 213:456
PMID: 26150546
Gofman et al. (2016) Relative Role of Akt, ERK and CREB in Alcohol-Induced Microglia P2X4R Receptor Expression. Alcohol Alcohol. 51:647
PMID: 26946194
Peng et al. (2016) Purinergic and store-operated Ca(²⁺) signaling mechanisms in mesenchymal stem cells and their roles in ATP-induced stimulation of cell migration. Stem Cells. 34:2102
PMID: 27038239
Kawano et al. (2015) Autocrine Regulation of UVA-Induced IL-6 Production via Release of ATP and Activation of P2Y Receptors. PLoS One. 10:e0127919
PMID: 26030257
Mascanfroni et al. (2015) Metabolic control of type 1 regulatory T cell differentiation by AHR and HIF1-α. Circulation. 21:638
PMID: 26005855
Maslieieva and Thompson (2014) A critical role for pannexin-1 in activation of innate immune cells of the choroid plexus. Channels (Austin). 8:131
PMID: 24418937
Ilatovskaya et al. (2013) Pharmacological characterization of the P2 receptors profile in the podocytes of the freshly isolated rat glomeruli. Am J Physiol Cell Physiol. 305:C1050
PMID: 24048730
Shabir et al. (2013) Functional expression of purinergic P2 receptors and transient receptor potential channels by the human urothelium. Nat Med. 305:F396
PMID: 23720349
Vergani et al. (2013) Long-term heart transplant survival by targeting the ionotropic purinergic receptor P2X7. J Biol Chem. 127:463
PMID: 23250993
Trabanelli et al. (2012) Extracellular ATP exerts opposite effects on activated and regulatory CD4+ T cells via purinergic P2 receptor activation. J Immunol. 189:1303
PMID: 22753942
Nörenberg et al. (2012) Positive allosteric modulation by iverm. of human but not murine P2X7 receptors. Br J Pharmacol. 167:48
PMID: 22506590
Wu et al. (2011) Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter. Am J Physiol Heart Circ Physiol. 301:H69
PMID: 21460192

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Customer Reviews for 5-BDBD (1)

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Name: Stefan Uderhardt

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: phagocyte

DMSO dilution 10 μM

PMID: PMID: 30955887

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Product Documents for 5-BDBD

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.