27-Hydroxycholesterol

Name: 27-Hydroxycholesterol
Brand: Tocris Bioscience
SKU: 3907

Tocris Bioscience a Bio-Techne Brand

Catalog # 3907

Citations (2)

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Key Product Details

Description:	LXR agonist; also ER partial agonist
Alternative Names:	27 OHC, 27 HC
Chemical Name:	(3β,25R)-Cholest-5-ene-3,26-diol
Purity:	≥98% (HPLC)
Molecular Weight:	402.65

View Full Technical Details for 27-Hydroxycholesterol

Biological Activity for 27-Hydroxycholesterol

27-Hydroxycholesterol is a liver X receptor (LXR) agonist. Also estrogen receptor partial agonist. Suppresses β-estradiol-mediated breast cancer cell proliferation in vitro. Also increases α-synuclein levels and induces apoptosis in human neuroblastoma cells in vitro. Endogenous cholesterol metabolite.

Technical Data for 27-Hydroxycholesterol

M. Wt	402.65
Formula	C₂₇H₄₆O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	20380-11-4
PubChem ID	123976
InChi Key	FYHRJWMENCALJY-YSQMORBQSA-N
Smiles	O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC[C@@H](C)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 27-Hydroxycholesterol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	ethanol	8.05	20

Preparing Stock Solutions for 27-Hydroxycholesterol

The following data is based on the product molecular weight 402.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	12.42 mL	62.09 mL	124.18 mL
1 mM	2.48 mL	12.42 mL	24.84 mL
2 mM	1.24 mL	6.21 mL	12.42 mL
10 mM	0.25 mL	1.24 mL	2.48 mL

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Reconstitution Calculator

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References for 27-Hydroxycholesterol

References are publications that support the biological activity of the product.

Rantham Prabhakara Differential effects of 24-hydroxycholesterol and 27-hydroxycholesterol on tyrosine hydroxylase and α-synuclein in human neuroblastoma SH-SY5Y cells. J.Neurochem. 2008 PMID: 19014385
DuSell 27-hydroxycholesterol is an endogenous selective estrogen receptor modulator Mol.Endocrinol. 2008 PMID: 17872378
Fu 27-hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells. J.Biol.Chem. 2001 PMID: 11504730

Citations for 27-Hydroxycholesterol (2)

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Citations are publications that use Bio-Techne products. Selected citations for 27-Hydroxycholesterol include:

Gibson et al. (2018) The impact of 27-hydroxycholesterol on endometrial cancer proliferation. Endocr Relat Cancer. 25:381
PMID: 29371332
Schommer et al. (2018) 27-Hydroxycholesterol increases α-synuclein protein levels through proteasomal inhibition in human dopaminergic neurons. BMC Neurosci. 19:17
PMID: 29614969

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