2-APB

Name: 2-APB
Brand: Tocris Bioscience
SKU: 1224
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1224

(3)

Citations (392)

COA / SDS

IP3 receptor antagonist. Also TRP channel modulator

Key Product Details

Description:	IP₃ receptor antagonist. Also TRP channel modulator
Chemical Name:	2-Aminoethoxydiphenylborane
Purity:	≥98% (HPLC)
Molecular Weight:	225.1

View Full Technical Details for 2-APB

Technical Data
Citations / Reviews (3)

Biological Activity for 2-APB

2-APB is a functional and membrane permeable D-myo-inositol 1,4,5-trisphosphate (IP₃) receptor antagonist (IC₅₀ = 42 μM). Stimulates store-operated calcium (SOC) release at low concentrations (< 10 μM) and inhibits it at higher concentrations (up to 50 μM). Increases STIM-Orai channel conductance and limits ion selectivity. Modulator of TRP channels; blocks TRPC1, TRPC3, TRPC5, TRPC6, TRPV6, TRPM3, TRPM7, TRPM8 and TRPP2 and at higher concentrations stimulates TRPV1, TRPV2 and TRPV3. Also blocks specific gap channel subtypes.

Technical Data for 2-APB

M. Wt	225.1
Formula	C₁₄H₁₆BNO
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	524-95-8
PubChem ID	1598
InChi Key	BLZVCIGGICSWIG-UHFFFAOYSA-N
Smiles	NCCOB(C1=CC=CC=C1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 2-APB

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	22.51	100 with gentle warming

Preparing Stock Solutions for 2-APB

The following data is based on the product molecular weight 225.1.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.44 mL	22.21 mL	44.42 mL
5 mM	0.89 mL	4.44 mL	8.88 mL
10 mM	0.44 mL	2.22 mL	4.44 mL
50 mM	0.09 mL	0.44 mL	0.89 mL

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Reconstitution Calculator

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References for 2-APB

References are publications that support the biological activity of the product.

Xu Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltafe-dependent effect. Br.J.Pharmacol. 2005 PMID: 15806115
Varnai STIM and Orai: the long-awaited constituents of store-operated calcium entry. TiPs 2009 PMID: 19187978
Maruyama 2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P₃-induced Ca²⁺ release. J.Biochem. 1997 PMID: 9348075
Togashi Inhibition of the transient receptor potential cation channel TRPM2 by 2-aminoethoxydiphenyl borate (2-APB). Br.J.Pharmacol. 2008 PMID: 18204483
Bai Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB). J.Pharmacol.Exp.Ther. 2006 PMID: 16985167