1400W dihydrochloride

Name: 1400W dihydrochloride
Brand: Tocris Bioscience
SKU: 1415
Rating: 5 (3 reviews)

Catalog # 1415 | Tocris Bioscience a Bio-Techne Brand

(3)

Citations (9)

COA / SDS

Potent, highly selective iNOS inhibitor

Key Product Details

Description:	Potent, highly selective iNOS inhibitor
Chemical Name:	N-[[3-(Aminomethyl)phenyl]methyl]-ethanimidamide dihydrochloride
Purity:	≥99% (HPLC)
Molecular Weight:	250.17

View Full Technical Details for 1400W dihydrochloride

Technical Data
Citations (9) / Reviews (3)

Biological Activity for 1400W dihydrochloride

1400W dihydrochloride is a slow, tight binding, potent and highly selective inhibitor of inducible nitric oxide synthase (K_d = 7 nM). Selective over nNOS and eNOS (K_i values are 2 and 50 μM respectively). Cell-permeable and active in vivo. Neuroprotective in epilepsy models and has analgesic effects in models of mechanical and heat hypersensitivity.

Technical Data for 1400W dihydrochloride

M. Wt	250.17
Formula	C₁₀H₁₅N₃.2HCl
Storage	Desiccate at RT
Purity	≥99% (HPLC)
CAS Number	214358-33-5
PubChem ID	2733515
InChi Key	WDJHSQZCZGPGAA-UHFFFAOYSA-N
Smiles	Cl.Cl.CC(=N)NCC1=CC=CC(CN)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 1400W dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	25.02	100

Preparing Stock Solutions for 1400W dihydrochloride

The following data is based on the product molecular weight 250.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.00 mL	19.99 mL	39.97 mL
5 mM	0.80 mL	4.00 mL	7.99 mL
10 mM	0.40 mL	2.00 mL	4.00 mL
50 mM	0.08 mL	0.40 mL	0.80 mL

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References for 1400W dihydrochloride

References are publications that support the biological activity of the product.

Putra Inducible nitric oxide synthase inhibitor, 1400W, mitigates DFP-induced long-term neurotoxicity in the rat model. Neurobiol.Dis. 2020 PMID: 30940499
Staunton Inducible nitric oxide synthase inhibition by 1400W limits pain hypersensitivity in a neuropathic pain rat model. Exp.Physiol 2018 PMID: 29441689
Parmentier Selective inhibition of inducible nitric oxide synthase prevents ischaemic brain injury. Br.J.Pharmacol. 1999 PMID: 10385257
Garvey 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J.Biol.Chem. 1997 PMID: 9030556
Thomsen Selective inhibition of inducible nitric oxide inhibits tumor growth in vivo: studies with 1400W, a novel inhibitor. Cancer Res. 1997 PMID: 9242464

Citations for 1400W dihydrochloride (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for 1400W dihydrochloride include:

M?ller et al. (2018) Both Type I and Type II Interferons Can Activate Antitumor M1 Macrophages When Combined With TLR Stimulation. Front Immunol. 9:2520
PMID: 30450098
Staunton et al. (2018) Inducible nitric oxide synthase inhibition by 1400W limits pain hypersensitivity in a neuropathic pain rat model Exp Physiol. 103:535
PMID: 29441689
Kuen et al. (2017) Pancreatic cancer cell/fibroblast co-culture induces M2 like macrophages that influence therapeutic response in a 3D model. PLoS One. 12:e0182039
PMID: 28750018
Clark et al. (2015) Selective activation of microglia facilitates synaptic strength. RNA. 35:4552
PMID: 25788673
Gross et al. (2013) Roles of endothelial nitric oxide synthase (eNOS) and mitochondrial permeability transition pore (MPTP) in epoxyeicosatrienoic acid (EET)-induced cardioprotection against infarction in intact rat hearts. J Mol Cell Cardiol. 59:20
PMID: 23419451
Gresa-Arribas et al. (2012) Modelling neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents. PLoS One. 7:e45227
PMID: 23028862
Al-Shalmani et al. (2011) Quercetin and its principal metabolites, but not myricetin, oppose lipopolysaccharide-induced hyporesponsiveness of the porcine isolated coronary artery. Br J Pharmacol. 162:1485
PMID: 21375526
Tsuda et al. (2009) Mechanisms underlying fibronectin-induced up-regulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signalling pathways. J Cell Mol Med. 13:3251
PMID: 19298529
Ashrafpour et al. (2004) Vasodilator effect and mechanism of action of vascular endothelial growth factor in skin vasculature. Am J Physiol Heart Circ Physiol. 286:H946
PMID: 14644765

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Customer Reviews for 1400W dihydrochloride (3)

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Inhibition of iNOS by 1400W

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: primary BMDM

Macrophages were treated with LPS (10 ng/mL) and IFN-y (20 ng/mL) for 24h in the presence of 1400W (100 uM)
1400W inhibits iNOS expression induced by LPS

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Macrophages

RAW macrophages were treated with 200 ng/ml LPS alone or after pre-treatment with 1 μM 1400W (30 min pre-incubation) for 8 h to follow iNOS expression using Western blot. LPS treatment elevated iNOS expression while 1400W pretreatment significantly blunted this effect.
iNOS inhibitor

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Used as iNOS inhibitor for organ bath experiments with rat and mouse aorta

Good solubility and stability

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Product Documents for 1400W dihydrochloride

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.