LDV FITC
Tocris Bioscience, part of Bio-Techne | Catalog # 4577
Fluorescent ligand for α4β1 (VLA-4)
Key Product Details
Description
Fluorescent ligand for α4β1 (VLA-4)
Product Description
LDV FITC is a fluorescent ligand that binds to the α4β1 integrin (VLA-4) with high affinity (Kd values are 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn2+ respectively). Used to detect VLA-4 affinity and conformational changes. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.Optical Data
| Application | TR-FRET, Confocal Microscopy |
Spectra Viewer
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Spectra ViewerProduct Specifications
Molecular Weight
1368.54
Formula
C69H81N11O17S
Storage
Store at -20°C
Purity
≥90% (HPLC)
CAS Number
1207610-07-8
PubChem ID
91826076
InChI Key
PAAUNHNWVWSJKI-CBRXRBRLSA-N
SMILES
CC(C)C[C@H](NC(=O)Cc1ccc(NC(=O)Nc2ccccc2C)cc1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCNC(=S)Nc1ccc(C(O)=O)c(c1)-c1c-2ccc(=O)cc-2oc2cc(O)ccc12)C(O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble to 1mg/ml in PBS (pH 7.4) |
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Background References
References are publications that support the biological activity of the product.
- Njus Conformational mAb as a tool for integrin ligand discovery. Assay Drug Dev.Technol. 2009 PMID: 19754304
- Chigaev FRET detection of cellular α4-integrin conformational activation. Biophys.J. 2003 PMID: 14645084
- Chigaev Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn2+ or antibody activation. J.Biol.Chem. 2001 PMID: 11641394
Product Specific Notices for LDV FITC
For research use only
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