Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-RP-000548
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIM-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each TIM-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for rat TIM-1.
Cell Culture Supernates, Rat EDTA Plasma, Rat Serum, Rat Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 19 |
| Mean (pg/mL) | 53.5 | 2476 | 50.9 | 2593 |
| Standard Deviation | 2.80 | 176 | 4.57 | 214 |
| CV% | 5.2 | 7.1 | 9.0 | 8.3 |
Recovery for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge
Recovery for the rat TIM-1 assays was evaluated at three different spiked concentrations of TIM-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Rat EDTA Plasma (n=4) | 102 | 94-113 |
| Rat Serum (n=4) | 103 | 94-111 |
| Rat Urine (n=4) | 110 | 103-115 |
Linearity
Samples containing and/or spiked with high concentrations of rat TIM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge
Simple Plex Rat TIM-1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Rat TIM-1 Assay. Assay range is 7.06-10,770 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.
Long Name
Alternate Names
Gene Symbol
Additional TIM-1/KIM-1/HAVCR Products
Product Documents for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge
Product Specific Notices for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge
For research use only