Simple Plex Mouse IL-17 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-003352
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse IL-17 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-17 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-17 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-17.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 56 | 56 |
| Mean (pg/mL) | 30.3 | 1561 | 35.8 | 1761 |
| Standard Deviation | 1.34 | 41.0 | 2.76 | 144 |
| CV% | 4.4 | 2.6 | 7.7 | 8.2 |
Recovery for Simple Plex Mouse IL-17 Cartridge
Recovery for the mouse IL-17 assays was evaluated at three different spiked concentrations of IL-17 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 98 | 90-105 |
| Heparin Plasma (n=4) | 109 | 99-116 |
| Serum (n=4) | 97 | 88-105 |
Linearity
Samples containing and/or spiked with high concentrations of mouse IL-17 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse IL-17 Cartridge
Simple Plex Mouse IL-17 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-17 Assay. Assay range is 1.50-5715 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-17/IL-17A
IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-17/IL-17A Products
Product Documents for Simple Plex Mouse IL-17 Cartridge
Product Specific Notices for Simple Plex Mouse IL-17 Cartridge
For research use only