Simple Plex Human Vitamin D BP Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000541
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Vitamin D BP Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Vitamin D BP assay.
Inter-Assay Precision (Precision between assays) Replicates of each Vitamin D BP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Vitamin D BP.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 51 | 54 |
| Mean (pg/mL) | 293 | 14947 | 311 | 18273 |
| Standard Deviation | 20.3 | 820 | 29.5 | 952 |
| CV% | 6.9 | 5.5 | 9.5 | 5.2 |
Recovery for Simple Plex Human Vitamin D BP Cartridge
Recovery was not necessary as human Vitamin D BP shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Vitamin D BP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Vitamin D BP Cartridge
Simple Plex Human Vitamin D BP Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Vitamin D BP Assay. Assay range is 232-56,710 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Vitamin D BP
Vitamin D binding protein (VitD BP), also known as DBP and Gc-globulin, is a 58 kDa glycoprotein that circulates at high concentration in the serum and serves as a carrier protein for vitamin D. The transport of vitamin D by VitD BP is important for the function of a wide variety of tissues. VitD BP binds both the 25(OH) and the hormonally active 1,25(OH)2 forms of vitamin D. VitD BP is primarily expressed in hepatocytes and to a lesser extent in the kidney. It delivers vitamin D into cells by Megalin-mediated endocytosis. A selectively deglycosylated form of VitD BP known as macrophage activating factor (MAF) blocks the angiogenic effects of FGF basic, VEGF, and Angiopoietin 2. VitD BP enhances the chemotaxis of monocytes and neutrophils to the activated complement component C5a or C5a des Arg (a C-terminally processed form of C5a). The chemotactic cofactor property of VitD BP is eliminated by binding to 1,25(OH)2 vitamin D, but it is not altered by binding to 25(OH) vitamin D or actin.
Long Name
Alternate Names
Gene Symbol
Additional Vitamin D BP Products
Product Documents for Simple Plex Human Vitamin D BP Cartridge
Product Specific Notices for Simple Plex Human Vitamin D BP Cartridge
For research use only